概述

  • 产品名称Anti-CCDC11抗体
    参阅全部 CCDC11 一抗
  • 描述
    兔多克隆抗体to CCDC11
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (YSQRFGTVQR EVKGPTPKVV IVRSKPPKGQ GAEHHLERIR RSHQKHNAIL) of Human CCDC11 (NP_659457).

  • 阳性对照
    • 293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab84324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).Can be blocked with CCDC11 peptide (ab117880). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-CCDC11 antibody 图像

  • Anti-CCDC11 antibody (ab84324) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
  • ab84324 staining CCDC11 in human skin fibroblasts by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformladehyde, permeabilized using 0.25% Triton X-100/PBS, blocked with 5% serum for 30 minutes at 25°C and then incubated with ab84324 at 1µg/ml for 3 hours at 25°C. The secondary used was an FITC conjugated donkey anti-rabbit IgG (H+L) used at a 1/500 dilution. Nuclear staining with DAPI.

    See Abreview

Anti-CCDC11 antibody (ab84324)参考文献

This product has been referenced in:
  • Perles Z  et al. A human laterality disorder associated with recessive CCDC11 mutation. J Med Genet 49:386-90 (2012). ICC/IF ; Human . Read more (PubMed: 22577226) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Skin Fibroblasts)
Specification Skin Fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - 0.25% Triton X-100 in PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

提交于 Dec 28 2011

Thank you for your message which has been forwarded to me as my colleague Miia is currently away from the office. We do request that customers submit their Abreview before the expirey date in order to make use of this offer. However, we understand ...

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Thank you for contacting us. The blocking peptide for the Anti-CCDC11 antibody (ab84324) is ab117880. This was previously not in our catalogue but has now been added due to your request. This may not be possible in every case but if any of your...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"