概述

  • 产品名称Anti-cbx7抗体
    参阅全部 cbx7 一抗
  • 描述
    兔多克隆抗体to cbx7
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 201-251 (DADLAEGPPP WTPALPSSEV TVTDITANSI TVTFREAQAA EGFFRDRSGK F), of Human cbx7 (NP_783640.1).

  • 阳性对照
    • HeLa or 293T cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab91431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 28 kDa.
IP Use at 5-15 µg/mg of lysate.

靶标

  • 相关性Control of cell proliferation by Polycomb group proteins (PcG) is an important facet of cellular homeostasis and its disruption can promote tumorigenesis. CBX7 is a novel PcG protein which is found to control the growth of normal cells. CBX7 is a gene that encodes a Polycomb protein, as shown by sequence homology, its interaction with Ring1 and its localization to nuclear Polycomb bodies. CBX7 extends the lifespan of a wide range of normal human cells and immortalizes mouse fibroblasts by downregulating expression of the Ink4a/Arf locus. CBX7 does not inter-function or colocalize with Bmi1, and both can exert their actions independently of each other as shown by reverse genetics. CBX7 was highly expressed in three different prostate cancer cell lines and present at elevated levels in normal prostate. (Gil et al., 2004).
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • Cbx 7 antibody
    • CBX7 antibody
    • Chromobox homolog 7 antibody
    • Chromobox protein homolog 7 antibody

Anti-cbx7 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling cbx7 with ab91431 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-cbx7 antibody (ab91431) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 28 kDa


    Exposure time : 10 seconds
  • cbx7 was immunoprecipitated from HeLa whole cell lysate, using ab91431 at 10 µg/mg of lysate. 20% of the immunoprecipitae was loaded per lane for the subsequent Western blot and probed with ab91431 at 1 µg/ml (lane 1) or a control IgG (lane 2).
    Detetion: chemoluminescence with exposure time of 30 seconds.

Anti-cbx7 antibody (ab91431)参考文献

ab91431 has not yet been referenced specifically in any publications.

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