概述

  • 产品名称Anti-cbx7抗体
    参阅全部 cbx7 一抗
  • 描述
    兔多克隆抗体to cbx7
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Chimpanzee
  • 免疫原

    Synthetic peptide derived from within residues 150 - 250 of Human cbx7.

    (Peptide available as ab22423.)

  • 阳性对照
    • This antibody gave a positive signal in MEF1 whole cell lysate and HeLa nuclear extract.

性能

应用

Our Abpromise guarantee covers the use of ab31765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

靶标

  • 相关性Control of cell proliferation by Polycomb group proteins (PcG) is an important facet of cellular homeostasis and its disruption can promote tumorigenesis. CBX7 is a novel PcG protein which is found to control the growth of normal cells. CBX7 is a gene that encodes a Polycomb protein, as shown by sequence homology, its interaction with Ring1 and its localization to nuclear Polycomb bodies. CBX7 extends the lifespan of a wide range of normal human cells and immortalizes mouse fibroblasts by downregulating expression of the Ink4a/Arf locus. CBX7 does not inter-function or colocalize with Bmi1, and both can exert their actions independently of each other as shown by reverse genetics. CBX7 was highly expressed in three different prostate cancer cell lines and present at elevated levels in normal prostate. (Gil et al., 2004).
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • Cbx 7 antibody
    • CBX7 antibody
    • Chromobox homolog 7 antibody
    • Chromobox protein homolog 7 antibody

Anti-cbx7 antibody 图像

  • All lanes : Anti-cbx7 antibody (ab31765) at 1 µg/ml

    Lane 1 : Hela nuclear Lysate
    Lane 2 : Hela nuclear Lysate with Human cbx7 peptide (ab22423) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Secondary ab: Alexa Fluor Goat polyclonal to Rabbit IgG (700)

    Predicted band size : 28 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-cbx7 antibody (ab31765) at 1 µg/ml + MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-cbx7 antibody (ab31765)参考文献

This product has been referenced in:
  • Maertens GN  et al. Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation of the INK4a tumour suppressor. EMBO J 29:2553-65 (2010). WB ; Human . Read more (PubMed: 20601937) »
  • Gelsomina_Mansueto, Floriana_Forzati, Angelo_Ferra Identification of a New Pathway for Tumor Progression Genes & Cancer 1:210 (2010). Read more (PubMed: ) »

See all 2 Publications for this product

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