概述

  • 产品名称Anti-CBX4抗体
    参阅全部 CBX4 一抗
  • 描述
    兔多克隆抗体to CBX4
  • 特异性In immunofluoresence, this antibody detects the expected discrete nuclear structure that is termed the PcG body, corresponding to the known localisation of PC2. The antibody has been successfully used to detect FLAG-tagged transfected hPC2 (see picture). It detects a weak band that probably corresponds to endogenous PC2, however, a strong secondary band is also seen at 50kD in all cell lines thus far tested. This suggests that the antibody also reacts with another ubiquitous highly expressed protein.
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    GLQDSSTDNRAKLC

    , corresponding to amino acids 95 - 107 of Human PC2.

  • 常规说明


    PC2 and other members of the Drosophila Polycomb group gene family are part of a cellular memory system that is responsible for the inheritance of gene activity by progeny cells.

性能

应用

Our Abpromise guarantee covers the use of ab4189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 82 kDa (predicted molecular weight: 61 kDa).

靶标

  • 功能E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • 组织特异性Ubiquitous.
  • 通路Protein modification; protein sumoylation.
  • 序列相似性Contains 1 chromo domain.
  • 翻译后修饰Phosphorylated on Thr-497 by HIPK2 upon DNA damage; which enhances E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CBX 4 antibody
    • CBX4 antibody
    • Cbx4 chromobox homolog 4 (Drosophila Pc class) antibody
    • CBX4_HUMAN antibody
    • Chromobox homolog 4 (Pc class homolog, Drosophila) antibody
    • Chromobox homolog 4 antibody
    • Chromobox protein homolog 4 antibody
    • E3 SUMO protein ligase CBX 4 antibody
    • E3 SUMO protein ligase CBX4 antibody
    • E3 SUMO-protein ligase CBX4 antibody
    • hPc 2 antibody
    • hPc2 antibody
    • NBP 16 antibody
    • NBP16 antibody
    • NS5ATP1 binding protein 16 antibody
    • Pc 2 antibody
    • Pc class 2 homolog antibody
    • Pc class homolog antibody
    • Pc class homolog Drosophila antibody
    • PC2 antibody
    • Polycomb 2 homolog antibody
    see all

Anti-CBX4 antibody 图像

  • Immunofluorescent imaging of human cells (U2OS) with ab4189 reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localisation of PC2. This corresponds to results seen in Satijn et al.

    IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/200 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes.  All blocking and incubation steps carried out at 37 degrees C. Nuclei counterstained with Hoechst stain (blue).

  • All lanes : Anti-CBX4 antibody (ab4189)

    Lane 1 : HEK 293 lysate over-expressing DDDDK tagged hPC2
    Lane 2 : HEK 293 lysate


    Predicted band size : 61 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa (possible non-specific secondary antibody binding),49 kDa (possible non-specific secondary antibody binding),65 kDa (possible non-specific secondary antibody binding).

    This image is courtesy of Dr Ari Melnick, Albert Einstein College of Medicine

    ab4189 specifically recognises over expressed DDDDK tagged hPC2 in HEK293 cells (lane1). The lower bands are assigned to degradation products.

Anti-CBX4 antibody (ab4189)参考文献

This product has been referenced in:
  • MacPherson MJ  et al. The CTCF insulator protein is posttranslationally modified by SUMO. Mol Cell Biol 29:714-25 (2009). ICC/IF ; Human . Read more (PubMed: 19029252) »

See 1 Publication for this product

Product Wall

I'm sorry to hear you are having problems with ab4189, I would like to suggest a few modifications to your protocol: -try a different fixation step, I would suggest a 10min acetone or methanol (ice cold) fixation, it can be a very good fixative in ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"