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PC2 and other members of the Drosophila Polycomb group gene family are part of a cellular memory system that is responsible for the inheritance of gene activity by progeny cells.
Our Abpromise guarantee covers the use of ab4189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Detects a band of approximately 82 kDa (predicted molecular weight: 61 kDa).|
Immunofluorescent imaging of human cells (U2OS) with ab4189 reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localisation of PC2. This corresponds to results seen in Satijn et al.
IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/200 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees C. Nuclei counterstained with Hoechst stain (blue).
This image is courtesy of Dr Ari Melnick, Albert Einstein College of Medicineab4189 specifically recognises over expressed DDDDK tagged hPC2 in HEK293 cells (lane1). The lower bands are assigned to degradation products.
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