The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 21 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.
组织特异性Expressed in all adult and embryonic tissues.
序列相似性Contains 2 chromo domains.
翻译后修饰Not phosphorylated. Ubiquitinated.
细胞定位Nucleus. Unassociated with chromosomes during mitosis.