Anti-CBS抗体(ab96252)
Key features and details
- Rabbit polyclonal to CBS
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human, Pig, Zebrafish
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CBS抗体
参阅全部 CBS 一抗 -
描述
兔多克隆抗体to CBS -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human, Pig, Zebrafish
预测可用于: Mouse, Rat, Rabbit, Cow -
免疫原
Recombinant fragment corresponding to a region within amino acids 39-221 of Human CBS (AAH00440).
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阳性对照
- WB: HeLa, Molt-4 and Raji whole cell lysates IHC-P: OVCAR3 xenograft; ICC: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96252于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
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IHC-P |
1/100 - 1/1000.
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说明 |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
IHC-P
1/100 - 1/1000. |
靶标
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功能
Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury. -
组织特异性
In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney. -
通路
Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2. -
疾病相关
Defects in CBS are the cause of cystathionine beta-synthase deficiency (CBSD) [MIM:236200]. CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine. -
序列相似性
Belongs to the cysteine synthase/cystathionine beta-synthase family.
Contains 1 CBS domain. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 875 Human
- Entrez Gene: 12411 Mouse
- Entrez Gene: 24250 Rat
- Entrez Gene: 550524 Zebrafish
- Omim: 613381 Human
- SwissProt: P35520 Human
- SwissProt: Q91WT9 Mouse
- SwissProt: P32232 Rat
see all -
别名
- AI047524 antibody
- AI303044 antibody
- Beta thionase antibody
see all
图片
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All lanes : Anti-CBS antibody (ab96252) at 1/5000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : CBS Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 61 kDa -
All lanes : Anti-CBS antibody (ab96252) at 1/5000 dilution
Lane 1 : Molt-4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft using ab96252 at 1/500 dilution.
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All lanes : Anti-CBS antibody (ab96252) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Lane 4 : Zebrafish liver homogenate at 20 µg
Lane 5 : Human epithelial carcinoma cell line at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
HeLa cells stained for CBS (green) using ab96252 at 1/200 dilution in ICC/IF.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab96252 被引用在 10 文献中.
- Tian JJ et al. Upregulation of Nav1.7 by endogenous hydrogen sulfide contributes to maintenance of neuropathic pain. Int J Mol Med 46:782-794 (2020). PubMed: 32468069
- Bartman CM et al. Hydrogen sulfide, oxygen, and calcium regulation in developing human airway smooth muscle. FASEB J 34:12991-13004 (2020). PubMed: 32777143
- Coletti R et al. Nitric oxide acutely modulates hypothalamic and neurohypophyseal carbon monoxide and hydrogen sulphide production to control vasopressin, oxytocin and atrial natriuretic peptide release in rats. J Neuroendocrinol 31:e12686 (2019). PubMed: 30633838
- Chen Y et al. O-GlcNAcylated c-Jun antagonizes ferroptosis via inhibiting GSH synthesis in liver cancer. Cell Signal 63:109384 (2019). PubMed: 31394193
- Song W et al. Edoxaban improves venous thrombosis via increasing hydrogen sulfide and homocysteine in rat model. Mol Med Rep 16:7706-7714 (2017). PubMed: 28944880
- Zhang R et al. Excessive nNOS/NO/AMPK signaling activation mediated by the blockage of the CBS/H2S system contributes to oxygen-glucose deprivation-induced endoplasmic reticulum stress in PC12 cells. Int J Mol Med 40:549-557 (2017). PubMed: 28656194
- Lechpammer M et al. Upregulation of cystathionine β-synthase and p70S6K/S6 in neonatal hypoxic ischemic brain injury. Brain Pathol 27:449-458 (2017). PubMed: 27465493
- Espe M et al. Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon. Br J Nutr 112:1274-85 (2014). PubMed: 25196630
- Kondo K et al. H2S protects against pressure overload-induced heart failure via upregulation of endothelial nitric oxide synthase. Circulation 127:1116-27 (2013). WB ; Mouse . PubMed: 23393010
- Kundu S et al. Hydrogen sulfide deficiency and diabetic renal remodeling: role of matrix metalloproteinase-9. Am J Physiol Endocrinol Metab 304:E1365-78 (2013). Mouse . PubMed: 23632630