Anti-CBR1抗体[4E12] (ab111347)

概述

  • 产品名称Anti-CBR1抗体[4E12]
    参阅全部 CBR1 一抗
  • 描述
    小鼠单克隆抗体[4E12] to CBR1
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-277 of Human CBR1, purified from E. coli (NP_001748).

  • 阳性对照
    • HeLa, HepG2 and 293T cell lysates. IF/ICC: HepG2 cell line

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab111347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 30 kDa.
ELISA Use at an assay dependent concentration.

靶标

  • 功能NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
  • 序列相似性Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 15 hydroxyprostaglandin dehydrogenase [NADP+] antibody
    • 15-hydroxyprostaglandin dehydrogenase [NADP+] antibody
    • Carbonyl reductase [NADPH] 1 antibody
    • Carbonyl Reductase 1 antibody
    • CBR 1 antibody
    • CBR1 antibody
    • CBR1_HUMAN antibody
    • CRN antibody
    • NADPH dependent carbonyl reductase 1 antibody
    • NADPH-dependent carbonyl reductase 1 antibody
    • Prostaglandin 9 ketoreductase antibody
    • Prostaglandin 9-ketoreductase antibody
    • Prostaglandin E(2) 9 reductase antibody
    • Prostaglandin-E(2) 9-reductase antibody
    • SDR21C1 antibody
    see all

Anti-CBR1 antibody [4E12] 图像

  • ICC/IF image of ab111347 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab111347, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-CBR1 antibody [4E12] (ab111347) at 1/1000 dilution

    Lane 1 : HeLa Cell Lysate
    Lane 2 : HepG2 Cell Lysate
    Lane 3 : 293T Cell Lysate

    Lysates/proteins at 40 µg per lane.

    Developed using the ECL technique

    Predicted band size : 30 kDa

Anti-CBR1 antibody [4E12] (ab111347)参考文献

ab111347 has not yet been referenced specifically in any publications.

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