概述

  • 产品名称Anti-CBLN1抗体
    参阅全部 CBLN1 一抗
  • 描述
    兔多克隆抗体to CBLN1
  • 特异性ab92526 detects endogenous levels of total CBLN1 protein.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from an internal sequence of Human CBLN1.

  • 阳性对照
    • HUVEC cell extracts.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab92526 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab92526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: 1/40000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 21 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the formation and maintenance of parallel fiber and Purkinje cell synapses. When parallel fibers make contact with Purkinje spines, CBLN1 interaction with GRID2 triggers the recruitment of NRXN1 and secretory vesicles to the sites of contact. NRXN1-CBLN1-GRID2 signaling induces presynaptic morphological changes, which may further accumulate pre- and postsynaptic components to promote bidirectional maturation of parallel fiber - Purkinje cell functionnally active synapses by a positive feedback mechanism. Required for CBLN3 export from the endoplasmic reticulum and secretion.
      The cerebellin peptide exerts neuromodulatory functions. Directly stimulates norepinephrine release via the adenylate cyclase/PKA-dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release.
    • 组织特异性In the Purkinje cells postsynaptic structures. In the cerebellum, cerebellin is much less abundant than [des-Ser1]-cerebellin.
    • 序列相似性Contains 1 C1q domain.
    • 发展阶段Low at birth, the cerebellin concentration increases between day 5 and 15, and reaches peak values between day 21 and 56.
    • 翻译后修饰The proteolytic processing to yield cerebellin seems to occur either prior to the secretion by presynaptic neurons and subsequent oligomerization or in some other location after release of the mature protein.
    • 细胞定位Secreted. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • [des-Ser1]-cerebellin antibody
      • CBLN 1 antibody
      • CBLN1 antibody
      • CBLN1_HUMAN antibody
      • CER antibody
      • Cerebellin 1 antibody
      • Cerebellin 1 precursor antibody
      • Cerebellin1 antibody
      • Precerebellin antibody
      see all

    Anti-CBLN1 antibody 图像

    • All lanes : Anti-CBLN1 antibody (ab92526) at 1/500 dilution

      Lane 1 : HUVEC cell extracts
      Lane 2 : HUVEC cell extracts with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 21 kDa

    Anti-CBLN1 antibody (ab92526)参考文献

    ab92526 has not yet been referenced specifically in any publications.

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