Anti-CBL (phospho Y731)抗体[EP973Y] (ab52855)


  • 产品名称Anti-CBL (phospho Y731)抗体[EP973Y]
    参阅全部 CBL 一抗
  • 描述
    兔单克隆抗体[EP973Y] to CBL (phospho Y731)
  • 特异性ab52855 detects CBL phosphorylated on Tyrosine 731.
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Tyr731 of human CBL.

  • 阳性对照
    • Raji cell lysates.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


Our Abpromise guarantee covers the use of ab52855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 120 kDa (predicted molecular weight: 100 kDa).
IP 1/50.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    • 功能Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
    • 通路Protein modification; protein ubiquitination.
    • 疾病相关Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects.
    • 序列相似性Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
      Contains 1 RING-type zinc finger.
      Contains 1 UBA domain.
    • 结构域The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
      The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
    • 翻译后修饰Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
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    Anti-CBL (phospho Y731) antibody [EP973Y] 图像

    • All lanes : Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855) at 1/2000 dilution

      Lane 1 : Raji cell lysate untreated
      Lane 2 : Raji cell lysate treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 100 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)

    Anti-CBL (phospho Y731) antibody [EP973Y] (ab52855)参考文献

    This product has been referenced in:
    • Wang D  et al. Tespa1 is involved in late thymocyte development through the regulation of TCR-mediated signaling. Nat Immunol 13:560-8 (2012). Read more (PubMed: 22561606) »
    • Lee JO  et al. Metformin regulates glucose transporter 4 (GLUT4) translocation through AMP-activated protein kinase (AMPK)-mediated Cbl/CAP signaling in 3T3-L1 preadipocyte cells. J Biol Chem 287:44121-9 (2012). WB . Read more (PubMed: 23135276) »

    See all 3 Publications for this product

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