RabMAb

Anti-CBL (phospho Y674)抗体[EPR2227] (ab76536)

概述

  • 产品名称Anti-CBL (phospho Y674)抗体[EPR2227]
    参阅全部 CBL 一抗
  • 描述
    兔单克隆抗体[EPR2227] to CBL (phospho Y674)
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding tyrosine 674 of human CBL.

  • 阳性对照
    • Jurkat cell lysate treated with pervanadate. Permeabilized Jurkat cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

应用

Our Abpromise guarantee covers the use of ab76536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 100 kDa.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
    • 通路Protein modification; protein ubiquitination.
    • 疾病相关Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects.
    • 序列相似性Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
      Contains 1 RING-type zinc finger.
      Contains 1 UBA domain.
    • 结构域The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
      The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
    • 翻译后修饰Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
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      • Oncogene CBL2 antibody
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    Anti-CBL (phospho Y674) antibody [EPR2227] 图像

    • All lanes : Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Jurkat cell lysate treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size : 100 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized Jurkat cells, un-treated (green) or pervanadate treated (red) using ab76536 at a 1/20 dilution, and pervanadate-treated Jurkat cells using the same antibody pre-incubated with phospho-CBL peptide (blue) or non-phospho-CBL peptide (orange).

    Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)参考文献

    ab76536 has not yet been referenced specifically in any publications.

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