概述

  • 产品名称Anti-CBL (phospho Y674)抗体
    参阅全部 CBL 一抗
  • 描述
    兔多克隆抗体to CBL (phospho Y674)
  • 特异性Binds CBL only when phosphorylated at these sites: Human: Tyr674; Mouse: Tyr672; Rat: Tyr780
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthesized phosphopeptide derived from human CBL around the phosphorylation site of tyrosine 674 (A-I-YP-S-L).

  • 阳性对照
    • HepG2 cells extract
  • 常规说明Stable at -20°C for 1 year

性能

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应用

Our Abpromise guarantee covers the use of ab58508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 99 kDa.
ELISA 1/5000.

靶标

  • 功能Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
  • 通路Protein modification; protein ubiquitination.
  • 疾病相关Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects.
  • 序列相似性Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
    Contains 1 RING-type zinc finger.
    Contains 1 UBA domain.
  • 结构域The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
    The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
  • 翻译后修饰Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
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Anti-CBL (phospho Y674) antibody 图像

  • All lanes : Anti-CBL (phospho Y674) antibody (ab58508) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells, treated with Na2VO3 (0.3nM, 40mins)
    Lane 2 : Extracts from HepG2 cells, treated with Na2VO3 (0.3nM, 40mins), plus immunising peptide


    Predicted band size : 99 kDa

Anti-CBL (phospho Y674) antibody (ab58508)参考文献

This product has been referenced in:
  • Fernandes MS  et al. Novel oncogenic mutations of CBL in human acute myeloid leukemia that activate growth and survival pathways depend on increased metabolism. J Biol Chem 285:32596-605 (2010). WB . Read more (PubMed: 20622007) »

See 1 Publication for this product

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