Anti-Cbl-c抗体(ab34750)

概述

  • 产品名称
    Anti-Cbl-c抗体
  • 描述
    兔多克隆抗体to Cbl-c
  • 特异性
    No reactivity is expected with Cbl-a or Cbl-b.
  • 经测试应用
    适用于: ELISA, WB, IP, ICC/IFmore details
  • 种属反应性

    预测可用于: Mouse, Rat, Human, Chimpanzee
  • 免疫原

    Synthetic peptide:

    PRKPRNAQPKVRLLK

    , corresponding to amino acids 444-458 of Human Cbl-c

  • 阳性对照
    • Hek 293T cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab34750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000 - 1/50000.
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

靶标

  • 相关性
    Cblc belongs to a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation. Cblc is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitous). Cblc, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Cblc knock out mice show no obvious phenotype. Thus, the physiological role of Cblc is not known.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • Cas Br M (murine) ecotropic retroviral transforming sequence c antibody
    • Cas Br M (murine) ectropic retroviral transforming sequence c antibody
    • Casitas B lineage lymphoma c antibody
    • CBL 3 antibody
    • Cbl proto-oncogene C, E3 ubiquitin protein ligase antibody
    • Cbl proto-oncogene, E3 ubiquitin protein ligase C antibody
    • CBL SL antibody
    • E3 ubiquitin-protein ligase CBL-C antibody
    • RING finger protein 57 antibody
    • RNF57 antibody
    • SH3 binding protein CBL C antibody
    • SH3-binding protein CBL-3 antibody
    • Signal transduction protein CBL C antibody
    see all

图片

  • All lanes : Anti-Cbl-c antibody (ab34750) at 1/500 dilution

    Lane 1 : Hek 293T cell lysate transfected with empty vector
    Lane 2 : Hek 293T cell lysate transfected with Cbl-c.
    Lane 3 : Hek 293T cell lysate transfected with empty vector
    Lane 4 : Hek 293T cell lysate transfected with Cbl-c.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Image courtesy of Human Protein Atlas
    ab34750 (1/500) showing strong nuclear and cytoplasmic stainning of cells of the kidney tubuli. Paraffin embedded human kidney tissue was incubated with ab123 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

文献

ab34750 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (7.5)
Sample
Mouse Tissue lysate - whole (Intestine Brain mammary gland)
Specification
Intestine Brain mammary gland
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Neha Zutshi

Verified customer

提交于 Jan 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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