概述

  • 产品名称Anti-CBL抗体[YE323]
    参阅全部 CBL 一抗
  • 描述
    兔单克隆抗体[YE323] to CBL
  • 经测试应用适用于: ICC, WB, Flow Cyt, IPmore details
    不适用于: IHC
  • 种属反应性
    与反应: Human
    预测可用于: Chicken
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CBL aa 850 to the C-terminus (C terminal).
    Database link: P22681

  • 表位ab32027 reacts with an epitope located in the C terminal region of CBL.
  • 阳性对照
    • Jurkat cell lysates, Hela Cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

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应用

Our Abpromise guarantee covers the use of ab32027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/100.
WB 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 99 kDa).
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/100.
  • 应用说明Is unsuitable for IHC.
  • 靶标

    • 功能Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
    • 通路Protein modification; protein ubiquitination.
    • 疾病相关Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects.
    • 序列相似性Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
      Contains 1 RING-type zinc finger.
      Contains 1 UBA domain.
    • 结构域The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
      The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
    • 翻译后修饰Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4732447J05Rik antibody
      • C CBL antibody
      • Cas Br M (murine) ecotropic retroviral transforming sequence antibody
      • Casitas B lineage lymphoma proto oncogene antibody
      • Casitas B-lineage lymphoma proto-oncogene antibody
      • CBL 2 antibody
      • cbl antibody
      • CBL_HUMAN antibody
      • CBL2 antibody
      • E3 ubiquitin protein ligase CBL antibody
      • E3 ubiquitin-protein ligase CBL antibody
      • Oncogene CBL2 antibody
      • Proto oncogene c CBL antibody
      • Proto-oncogene c-CBL antibody
      • RGD1561386 antibody
      • RING finger protein 55 antibody
      • RNF55 antibody
      • Signal transduction protein CBL antibody
      see all

    Anti-CBL antibody [YE323] 图像

    • Anti-CBL antibody [YE323] (ab32027) at 1/5000 dilution + Jurkat cell lysates

      Predicted band size : 99 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of HeLa cells using 1/100 anti-Cbl (C-term) ab32027.

    Anti-CBL antibody [YE323] (ab32027)参考文献

    This product has been referenced in:
    • Walsh AM & Lazzara MJ Regulation of EGFR trafficking and cell signaling by Sprouty2 and MIG6 in lung cancer cells. J Cell Sci 126:4339-48 (2013). WB ; Human . Read more (PubMed: 23868981) »

    See 1 Publication for this product

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