概述

  • 产品名称Anti-CBFb抗体
    参阅全部 CBFb 一抗
  • 描述
    兔多克隆抗体to CBFb
  • 特异性This antibody detects CBFb.
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Zebrafish
    预测可用于: Chicken, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    MPRVVPDQRSKFENEEFFRK

    , corresponding to amino acids 1-20 of Human CBFb.

  • 阳性对照
    • HeLa whole cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: PBS, 1mg/ml BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab33516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500. PubMed: 24648201
WB 1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

靶标

  • 功能CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1.
  • 疾病相关Note=A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11.
  • 序列相似性Belongs to the CBF-beta family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CBF b antibody
    • CBF beta antibody
    • CBF-beta antibody
    • CBFB antibody
    • CBFbeta antibody
    • Core binding factor beta subunit antibody
    • core binding factor subunit beta antibody
    • Core-binding factor subunit beta antibody
    • PEA 2 antibody
    • PEA2 antibody
    • PEA2 beta antibody
    • PEA2-beta antibody
    • PEA2beta antibody
    • PEBB_HUMAN antibody
    • PEBP 2B antibody
    • PEBP2 beta antibody
    • PEBP2-beta antibody
    • PEBP2B antibody
    • PEBP2beta antibody
    • Polyomavirus enhancer binding protein 2 beta subunit antibody
    • Polyomavirus enhancer-binding protein 2 beta subunit antibody
    • SL3 3 enhancer factor 1 beta subunit antibody
    • SL3 3 enhancer factor 1 subunit beta antibody
    • SL3-3 enhancer factor 1 subunit beta antibody
    • SL3/AKV core binding factor beta subunit antibody
    • SL3/AKV core-binding factor beta subunit antibody
    see all

Anti-CBFb antibody (ab33516)参考文献

This product has been referenced in:
  • Cieslak A  et al. RUNX1-dependent RAG1 deposition instigates human TCR-d locus rearrangement. J Exp Med 211:1821-32 (2014). WB, IP ; Human . Read more (PubMed: 25135298) »
  • Lopez-Camacho C  et al. Core binding factor ß (CBFß) is retained in the midbody during cytokinesis. J Cell Physiol 229:1466-74 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24648201) »

See all 7 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Embryonic stem cells Strain 129)
Loading amount 35 µg
Specification Mouse Embryonic stem cells Strain 129
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Oct 04 2010

Application Western blot
Sample Rat Cell lysate - whole cell (UMR-106 OSTEOSARCOMA)
Loading amount 50 µg
Specification UMR-106 OSTEOSARCOMA
Gel Running Conditions Reduced Denaturing (10% PAGE SDS)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. CESAR CAMACHO

Verified customer

提交于 May 06 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"