概述

  • 产品名称
    Anti-CBFb抗体
    参阅全部 CBFb 一抗
  • 描述
    兔多克隆抗体to CBFb
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Horse, Dog, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 166-187 of Human CBFb (NP_074036.1).

  • 阳性对照
    • Jurkat whole cell lysate HeLa whole cell lysate 293T whole cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab125191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 22 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能
    CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1.
  • 疾病相关
    Note=A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11.
  • 序列相似性
    Belongs to the CBF-beta family.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • CBF b antibody
      • CBF beta antibody
      • CBF-beta antibody
      • CBFB antibody
      • CBFbeta antibody
      • Core binding factor beta subunit antibody
      • core binding factor subunit beta antibody
      • Core-binding factor subunit beta antibody
      • PEA 2 antibody
      • PEA2 antibody
      • PEA2 beta antibody
      • PEA2-beta antibody
      • PEA2beta antibody
      • PEBB_HUMAN antibody
      • PEBP 2B antibody
      • PEBP2 beta antibody
      • PEBP2-beta antibody
      • PEBP2B antibody
      • PEBP2beta antibody
      • Polyomavirus enhancer binding protein 2 beta subunit antibody
      • Polyomavirus enhancer-binding protein 2 beta subunit antibody
      • SL3 3 enhancer factor 1 beta subunit antibody
      • SL3 3 enhancer factor 1 subunit beta antibody
      • SL3-3 enhancer factor 1 subunit beta antibody
      • SL3/AKV core binding factor beta subunit antibody
      • SL3/AKV core-binding factor beta subunit antibody
      see all

    图片

    • Western blot - Anti-CBFb antibody (ab125191)
      Western blot - Anti-CBFb antibody (ab125191)
      All lanes : Anti-CBFb antibody (ab125191) at 0.1 µg/ml

      Lane 1 : Jurkat whole cell lysate at 50 µg
      Lane 2 : Jurkat whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 50 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 22 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-CBFb antibody (ab125191)
      Immunoprecipitation - Anti-CBFb antibody (ab125191)
      Immunoprecipitation : 1 mg of Jurkat cell lysate immunoprecipitated with ab125191 at 6 µg/mg lysate; 20% of immunoprecipitate loaded in lane

      Western Blot: CBFb antibody (ab125191) at 1 µg/ml

      developed using the ECL technique

      Exposure time : 30 seconds

    文献

    ab125191 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab125191？请让我们知道，以便我们可以引用本数据表中的参考文章。

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