概述

  • 产品名称
  • 描述
    兔多克隆抗体to CBFb
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Horse, Dog, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 166-187 of Human CBFb (NP_074036.1).

  • 阳性对照
    • Jurkat whole cell lysate HeLa whole cell lysate 293T whole cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab125191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 22 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能
    CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1.
  • 疾病相关
    Note=A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11.
  • 序列相似性
    Belongs to the CBF-beta family.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CBF b antibody
    • CBF beta antibody
    • CBF-beta antibody
    • CBFB antibody
    • CBFbeta antibody
    • Core binding factor beta subunit antibody
    • core binding factor subunit beta antibody
    • Core-binding factor subunit beta antibody
    • PEA 2 antibody
    • PEA2 antibody
    • PEA2 beta antibody
    • PEA2-beta antibody
    • PEA2beta antibody
    • PEBB_HUMAN antibody
    • PEBP 2B antibody
    • PEBP2 beta antibody
    • PEBP2-beta antibody
    • PEBP2B antibody
    • PEBP2beta antibody
    • Polyomavirus enhancer binding protein 2 beta subunit antibody
    • Polyomavirus enhancer-binding protein 2 beta subunit antibody
    • SL3 3 enhancer factor 1 beta subunit antibody
    • SL3 3 enhancer factor 1 subunit beta antibody
    • SL3-3 enhancer factor 1 subunit beta antibody
    • SL3/AKV core binding factor beta subunit antibody
    • SL3/AKV core-binding factor beta subunit antibody
    see all

图片

  • All lanes : Anti-CBFb antibody (ab125191) at 0.1 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 22 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation : 1 mg of Jurkat cell lysate immunoprecipitated with ab125191 at 6 µg/mg lysate; 20% of immunoprecipitate loaded in lane

    Western Blot: CBFb antibody (ab125191) at 1 µg/ml

    developed using the ECL technique

    Exposure time : 30 seconds

文献

ab125191 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab125191.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册