Anti-CBFb抗体(ab125191)
Key features and details
- Rabbit polyclonal to CBFb
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CBFb抗体
参阅全部 CBFb 一抗 -
描述
兔多克隆抗体to CBFb -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide, corresponding to a region within amino acids 166-187 of Human CBFb (NP_074036.1).
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阳性对照
- WB: HeLa, HEK-293T, TCMK, NIH/3T3 and Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125191于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 22 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 22 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1. -
疾病相关
Note=A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11. -
序列相似性
Belongs to the CBF-beta family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 865 Human
- Entrez Gene: 12400 Mouse
- Omim: 121360 Human
- SwissProt: Q13951 Human
- SwissProt: Q08024 Mouse
- Unigene: 460988 Human
- Unigene: 2018 Mouse
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别名
- CBF b antibody
- CBF beta antibody
- CBF-beta antibody
see all
图片
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All lanes : Anti-CBFb antibody (ab125191) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 22 kDa
Exposure time: 30 secondsCells prepared using NETN lysis buffer.
4-12% SDS-PAGE.
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Detection of human CBFb by western blot of immunoprecipitates.
Lane 1: Sample.
Lane 2: Control IgG.
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat (Human T cell leukemia cell line from peripheral blood) cells prepared using NETN lysis buffer.
Antibodies: ab125191 used for IP at 3 μg per reaction.For blotting immunoprecipitated CBFb ab125191 was used at 1 μg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab125191 被引用在 5 文献中.
- Simeoni F et al. Enhancer recruitment of transcription repressors RUNX1 and TLE3 by mis-expressed FOXC1 blocks differentiation in acute myeloid leukemia. Cell Rep 36:109725 (2021). PubMed: 34551306
- Malik N et al. The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription. Nat Commun 10:2071 (2019). PubMed: 31061501
- Shin MH et al. A RUNX2-Mediated Epigenetic Regulation of the Survival of p53 Defective Cancer Cells. PLoS Genet 12:e1005884 (2016). PubMed: 26925584
- Huang P et al. Direct reprogramming of human fibroblasts to functional and expandable hepatocytes. Cell Stem Cell 14:370-84 (2014). PubMed: 24582927
- Lopez-Camacho C et al. Core binding factor ß (CBFß) is retained in the midbody during cytokinesis. J Cell Physiol 229:1466-74 (2014). PubMed: 24648201