概述

  • 产品名称Anti-CBF抗体
    参阅全部 CBF 一抗
  • 描述
    小鼠多克隆抗体to CBF
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Full length human CBF protein (NP_005751.2).

  • 阳性对照
    • WB: NIH/3T3 cell lysate; K-562 cell lysate; CBF transfected 293T cell lysate ICC/IF: HeLa cell

性能

应用

Our Abpromise guarantee covers the use of ab89034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 121 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

Anti-CBF antibody 图像

  • Anti-CBF antibody (ab89034) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg

    Predicted band size : 121 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • Anti-CBF antibody (ab89034) at 1 µg/ml + K-562 cell lysate at 50 µg

    Predicted band size : 121 kDa
    Observed band size : 131 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CBF antibody (ab89034) at 1 µg/ml

    Lane 1 : CEBPZ transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 121 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
  • ab89034, at 10 µg/ml, staining CBF in Hela cell by Immunofluorescence.

Anti-CBF antibody (ab89034)参考文献

ab89034 has not yet been referenced specifically in any publications.

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