Anti-CBARA1抗体(ab102830)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to CBARA1
  • 特异性
    This antibody is expected to recognize isoform 1 and 2.
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a sequence between N terminal amino acids 59-89 of Human CBARA1.

  • 阳性对照
    • K562 cell line lysate IF/ICC: HepG2 cell line

性能

应用

Our Abpromise guarantee covers the use of ab102830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 54 kDa.

靶标

  • 功能
    Key regulator of mitochondrial calcium uptake required for calcium entry into mitochondrion. May act as a calcium sensor via its EF-hand domains, gating the activity of a calcium channel partner. Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG.
  • 组织特异性
    Expressed in epithelial cell line. Strongly expressed in epidermal keratinocytes and dermal endothelial cells.
  • 序列相似性
    Belongs to the MICU1 family.
    Contains 2 EF-hand domains.
  • 结构域
    The EF-hand domains are required for mitochondrial calcium homeostasis function.
  • 细胞定位
    Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Allergen Hom s 4 antibody
    • ara CALC antibody
    • atopy related autoantigen antibody
    • atopy related autoantigen CALC antibody
    • Atopy-related autoantigen CALC antibody
    • CALC antibody
    • calcium binding atopy related autoantigen 1 antibody
    • Calcium uptake protein 1 antibody
    • calcium uptake protein 1, mitochondrial antibody
    • Calcium-binding atopy-related autoantigen 1 antibody
    • CBARA1 antibody
    • EFHA3 antibody
    • FLJ12684 antibody
    • MICU1 antibody
    • MICU1_HUMAN antibody
    • mitochondrial antibody
    • mitochondrial calcium uptake 1 antibody
    • MPXPS antibody
    see all

Anti-CBARA1 antibody 图像

  • ICC/IF image of ab102830 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab102830, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CBARA1 antibody (ab102830) at 1/100 dilution + K562 cell line lysate at 35 µg

    Predicted band size : 54 kDa

Anti-CBARA1 antibody (ab102830)参考文献

ab102830 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

I am sorry that these antibodies did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passin...

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Thank you for submitting an Abreview for ab102830. As you may have noticed, your review has now been published on our website.

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - other (rat brain mitochondria)
Loading amount
35 µg
Specification
rat brain mitochondria
Gel Running Conditions
Non-reduced Denaturing
Blocking step
StartingBlock T20 (PBS) Blocking Buffer as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Username

Dr. Alexander Komarov

Verified customer

提交于 Aug 24 2012

Thank you for this information.


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab 135358.

To check the sta...

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Thank you for your reply.


The immunogen sequence used to generate this antibody (ab102830) is excluded from this isoform, so this is not a possibility. Ab115025 would recognize this isoform, but itis not present on your blot.

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Thank you for your reply.


Yes, this antibody is detecting Isoform 4 (MW 32kDa), as opposed to the other two which detected isoform 1 in the samples. You may still detect a 55kDa band in your samples if that is the isoform present.
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Thank you for contacting Abcam regarding these CBARA1 antibodies.


Based on the information and images you provided, I agree that these antibodies are not performing as we would expect. I am happy to offer replacements or refunds for both ...

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I am very pleased to hear you would like to accept our offer and test ab102830 in rat. This code will give you: 1 freePRIMARY ANTIBODYOR $337.00 OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for ab102830 ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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