概述

  • 产品名称Anti-Caveolin-3抗体
    参阅全部 Caveolin-3 一抗
  • 描述
    山羊多克隆抗体to Caveolin-3
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human Caveolin-3 aa 5-18 (N terminal) (Cysteine residue).
    Sequence:

    EHTDLEAQIVKDIH


    Database link: P56539

  • 阳性对照
    • Human, mouse and rat heart lysates.

性能

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应用

Our Abpromise guarantee covers the use of ab87770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 17 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress.
  • 组织特异性Expressed predominantly in muscle.
  • 疾病相关Defects in CAV3 are the cause of limb-girdle muscular dystrophy type 1C (LGMD1C) [MIM:607801]. LGMD1C is a myopathy characterized by calf hypertrophy and mild to moderate proximal muscle weakness. LGMD1C inheritance can be autosomal dominant or recessive.
    Defects in CAV3 are a cause of hyperCKmia (HYPCK) [MIM:123320]. It is a disease characterized by persistent elevated levels of serum creatine kinase without muscle weakness.
    Defects in CAV3 are a cause of rippling muscle disease (RMD) [MIM:606072]. RMD is a rare disorder characterized by mechanically triggered contractions of skeletal muscle. In RMD, mechanical stimulation leads to electrically silent muscle contractions that spread to neighboring fibers that cause visible ripples to move over the muscle.
    Defects in CAV3 are a cause of cardiomyopathy familial hypertrophic (CMH) [MIM:192600]; also designated FHC or HCM. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in CAV3 are the cause of long QT syndrome type 9 (LQT9) [MIM:611818]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy.
    Defects in CAV3 can be a cause of sudden infant death syndrome (SIDS) [MIM:272120]. SIDS is the sudden death of an infant younger than 1 year that remains unexplained after a thorough case investigation, including performance of a complete autopsy, examination of the death scene, and review of clinical history. Pathophysiologic mechanisms for SIDS may include respiratory dysfunction, cardiac dysrhythmias, cardiorespiratory instability, and inborn errors of metabolism, but definitive pathogenic mechanisms precipitating an infant sudden death remain elusive. Long QT syndromes-associated mutations can be responsible for some SIDS cases.
  • 序列相似性Belongs to the caveolin family.
  • 细胞定位Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAV3 antibody
    • CAV3_HUMAN antibody
    • Caveolin 3 antibody
    • Caveolin-3 antibody
    • LGMD1C antibody
    • LQT9 antibody
    • M-caveolin antibody
    • MGC126100 antibody
    • MGC126101 antibody
    • MGC126129 antibody
    • OTTHUMP00000115603 antibody
    • OTTHUMP00000207105 antibody
    • VIP 21 antibody
    • VIP21 antibody
    see all

Anti-Caveolin-3 antibody 图像

  • All lanes : Anti-Caveolin-3 antibody (ab87770) at 0.1 µg/ml

    Lane 1 : pig skeletal muscle tissue lysate
    Lane 2 : pig heart tissue lysate


    Predicted band size : 17 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Caveolin-3 antibody (ab87770) at 0.3 µg/ml

    Lane 1 : mouse heart tissue lysate
    Lane 2 : rat heart tissue lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 17 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
  • Anti-Caveolin-3 antibody (ab87770) at 0.3 µg/ml + human heart lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 17 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour
  • ab87770 (3.8µg/ml) staining of paraffin embedded Human Skeletal Muscle shows peripheral staining of fibres. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-Caveolin-3 antibody (ab87770)参考文献

ab87770 has not yet been referenced specifically in any publications.

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