Anti-Caveolin-2 (phospho Y27)抗体[EP2221Y] (ab76405)
Key features and details
- Rabbit monoclonal [EP2221Y] to Caveolin-2 (phospho Y27)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-Caveolin-2 (phospho Y27)抗体[EP2221Y]
参阅全部 Caveolin-2 一抗 -
描述
兔单克隆抗体[EP2221Y] to Caveolin-2 (phospho Y27) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa cell lysates treated with pervanadate
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP2221Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76405于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 18 kDa.
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说明 |
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WB
1/1000 - 1/2000. Predicted molecular weight: 18 kDa. |
靶标
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功能
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression. -
组织特异性
Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts. -
序列相似性
Belongs to the caveolin family. -
翻译后修饰
Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin. -
细胞定位
Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments. - Information by UniProt
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数据库链接
- Entrez Gene: 858 Human
- Omim: 601048 Human
- SwissProt: P51636 Human
- Unigene: 212332 Human
- Unigene: 603096 Human
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别名
- CAV antibody
- CAV2 antibody
- CAV2_HUMAN antibody
see all
图片
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All lanes : Anti-Caveolin-2 (phospho Y27) antibody [EP2221Y] (ab76405) at 1/2000 dilution
Lane 1 : HeLa cell lysates untreated.
Lane 2 : HeLa cell lysates treated with pervanadate.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab76405 尚未被引用在任何文献中。