RabMAb

Anti-Caveolin-2 (phospho Y19)抗体[EPR2220(3)] (ab124883)

概述

  • 产品名称Anti-Caveolin-2 (phospho Y19)抗体[EPR2220(3)]
    参阅全部 Caveolin-2 一抗
  • 描述
    兔单克隆抗体[EPR2220(3)] to Caveolin-2 (phospho Y19)
  • 特异性ab124883 detects Caveolin-2 phosphorylated at tyrosine 19.
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caveolin-2 aa 1-100.
    Database link: P51636

  • 阳性对照
    • HUVEC cell lysate treated with Pervanadate; HUVEC cells treated with Pervanadate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab124883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    • 功能May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
    • 组织特异性Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
    • 序列相似性Belongs to the caveolin family.
    • 翻译后修饰Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
    • 细胞定位Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CAV antibody
      • CAV2 antibody
      • CAV2_HUMAN antibody
      • Caveolae protein 20 kD antibody
      • Caveolin 2 antibody
      • Caveolin 2 isoform a and b antibody
      • Caveolin-2 antibody
      • MGC12294 antibody
      • OTTHUMP00000025032 antibody
      • OTTHUMP00000195982 antibody
      see all

    Anti-Caveolin-2 (phospho Y19) antibody [EPR2220(3)] 图像

    • All lanes : Anti-Caveolin-2 (phospho Y19) antibody [EPR2220(3)] (ab124883) at 1/1000 dilution

      Lane 1 : HUVEC cell lysate untreated
      Lane 2 : HUVEC cell lysate treated with Pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 18 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized HUVEC cells, untreated (green) or pervanadate treated (red) using ab124883 at 1/10 dilution, and pervanadate-treated HUVEC cells using the same antibody preincubated with phospho-Caveolin 2 Phospho (pY19) peptide (blue) or non-phospho- Caveolin-2 Phospho (pY19) peptide (orange).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Caveolin-2 (phospho Y19) antibody [EPR2220(3)] (ab124883)参考文献

    ab124883 has not yet been referenced specifically in any publications.

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