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RabMAb

Anti-Caveolin-2抗体[EPR2220] - Caveolae Marker (ab79397)

概述

  • 产品名称
    Anti-Caveolin-2抗体[EPR2220] - Caveolae Marker
    参阅全部 Caveolin-2 一抗
  • 描述
    兔单克隆抗体[EPR2220] to Caveolin-2 - Caveolae Marker
  • 经测试应用
    适用于: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caveolin-2.
    Database link: P51636

  • 阳性对照
    • WB: HeLa cell lysate. IHC-P: Human uterus tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • 常规说明

     

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab79397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/10000. Predicted molecular weight: 18 kDa.
IP 1/10.
IHC-P 1/100 - 1/250.
Flow Cyt 1/2000.
ICC/IF 1/100 - 1/250.

靶标

  • 功能
    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
  • 组织特异性
    Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
  • 序列相似性
    Belongs to the caveolin family.
  • 翻译后修饰
    Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
  • 细胞定位
    Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAV antibody
    • CAV2 antibody
    • CAV2_HUMAN antibody
    • Caveolae protein 20 kD antibody
    • Caveolin 2 antibody
    • Caveolin 2 isoform a and b antibody
    • Caveolin-2 antibody
    • MGC12294 antibody
    • OTTHUMP00000025032 antibody
    • OTTHUMP00000195982 antibody
    see all

Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker 图像

  • Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397) at 1/5000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 18 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
  • ab79397 at 1/100 dilution staining Caveolin-2 in human uterus by Immunohistochemistry using paraffin-embedded tissue. Note staining of endothelial cells and smooth muscles.

  • ab79397 at 1/100 dilution staining Caveolin-2 in HeLa cells by Immunofluorescence.

  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Caveolin-2 (red) with ab79397 at a 1/2000 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)参考文献

ab79397 has not yet been referenced specifically in any publications.

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Regarding antibodies ab79397 and ab32577, they both have been tested in IHC with paraffin embedded sections in the species stated on the datasheet. We don’t have preliminary data using them with frozen secti...

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