- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
-
产品名称Anti-Caveolin-2抗体
参阅全部 Caveolin-2 一抗 -
描述兔多克隆抗体to Caveolin-2
-
经测试应用适用于: ICC/IF, IHC-P, WBmore details
-
种属反应性与反应: Human
预测可用于: Rabbit, Chimpanzee, Monkey, Baboon, Gorilla, Orangutan
-
免疫原
-
阳性对照
- This antibody gave a positive signal in Human lung tissue lysate, and in the following whole cell lysates: HUVEC; WI38; A431.
性能
-
形式Liquid
-
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
-
克隆多克隆
-
同种型IgG
-
研究领域
相关产品
-
Compatible Secondaries
-
Positive Controls
应用
Our Abpromise guarantee covers the use of ab75865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/250. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). |
靶标
-
功能May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
-
组织特异性Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
-
序列相似性Belongs to the caveolin family.
-
翻译后修饰Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
-
细胞定位Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
- Information by UniProt
-
数据库链接
- Entrez Gene: 858 Human
- Omim: 601048 Human
- SwissProt: P51636 Human
- Unigene: 212332 Human
- Unigene: 603096 Human
-
别名
- CAV antibody
- CAV2 antibody
- CAV2_HUMAN antibody
see all
Anti-Caveolin-2 antibody 图像
-
Western blot - Anti-Caveolin-2 antibody (ab75865)All lanes : Anti-Caveolin-2 antibody (ab75865) at 1 µg/ml
Lane 1 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 2 :WI-38 whole cell lysate (ab3960)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 18 kDa
Observed band size : 18 kDa
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody (ab75865)ICC/IF image of ab75865 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75865, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2, and MCF-7 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, and MCF-7 cells at 5µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 antibody (ab75865)IHC image of Calveolin 2 staining in human cervix carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75865, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
实验方案
Anti-Caveolin-2 antibody (ab75865)参考文献
This product has been referenced in:
- Yamaguchi T et al. ROR1 sustains caveolae and survival signalling as a scaffold of cavin-1 and caveolin-1. Nat Commun 7:10060 (2016). Read more (PubMed: 26725982) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"