Anti-Caveolin-1 (phospho Y14)抗体(ab38468)

概述

  • 产品名称Anti-Caveolin-1 (phospho Y14)抗体
    参阅全部 Caveolin-1 一抗
  • 描述
    兔多克隆抗体to Caveolin-1 (phospho Y14)
  • 特异性This antibody is specific for Caveolin-1 only when phosphorylated at tyrosine 14.
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Cow, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human Caveolin-1.
    Database link: Q03135

  • 阳性对照
    • NIH 3T3 cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab38468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ELISA 1/20000.
ICC/IF 1/250.

靶标

  • 功能May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • 组织特异性Expressed in muscle and lung, less so in liver, brain and kidney.
  • 疾病相关Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • 序列相似性Belongs to the caveolin family.
  • 翻译后修饰The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • 细胞定位Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BSCL3 antibody
    • CAV antibody
    • CAV1 antibody
    • CAV1_HUMAN antibody
    • caveolae protein, 22 kD antibody
    • caveolin 1 alpha isoform antibody
    • caveolin 1 beta isoform antibody
    • Caveolin 1 caveolae protein 22kDa antibody
    • Caveolin-1 antibody
    • Caveolin1 antibody
    • cell growth-inhibiting protein 32 antibody
    • CGL3 antibody
    • LCCNS antibody
    • MSTP085 antibody
    • OTTHUMP00000025031 antibody
    • PPH3 antibody
    • VIP 21 antibody
    • VIP21 antibody
    see all

Anti-Caveolin-1 (phospho Y14) antibody 图像

  • Lanes 1 - 2 : Caveolin-1 Antibody
    Lanes 3 - 4 : Anti-Caveolin-1 (phospho Y14) antibody (ab38468) at 1/500 dilution

    Lane 1 : NIH 3T3 cell extract
    Lane 2 : NIH 3T3 cell extract. Pre-incubated with synthesized peptide
    Lane 3 : NIH 3T3 cell extract.
    Lane 4 : NIH 3T3 cell extract. Treated with H2O2.


    Predicted band size : 20 kDa
    Observed band size : 20 kDa

Anti-Caveolin-1 (phospho Y14) antibody (ab38468)参考文献

ab38468 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (uterus)
Specification uterus
Fixative no fixation
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 07 2012

Thanks you for your email.

This antibody should bind to reduced or denatured form, because we got positive binding for Western blots with the lysates of HuvEc and Hela cells. We haven’t received complains about this antibody. We didn&rs...

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Thank you for your email. I am sorry to hear that you are experiencing problems with this antibody.

I have read the protocol which looks fine to me however I would like to suggest few changes which will help to understand the cause of the pro...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Cell lysate - whole cell (Bovine Brain microvascular endothelial cells)
Loading amount 40 µg
Specification Bovine Brain microvascular endothelial cells
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Prof. JL Balligand

Verified customer

提交于 Feb 11 2011

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