RabMAb

Anti-Caveolin-1抗体[E249] - Caveolae Marker (ab32577)

概述

  • 产品名称Anti-Caveolin-1抗体[E249] - Caveolae Marker
    参阅全部 Caveolin-1 一抗
  • 描述
    兔单克隆抗体[E249] to Caveolin-1 - Caveolae Marker
  • 特异性This antibody should recognize both alpha and beta form of Caveolin-1.
  • 经测试应用适用于: ICC/IF, IHC-P, ICC, WBmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caveolin-1 aa 150-250.
    Database link: Q03135

  • 阳性对照
    • A431 cell lysate, human urinary bladder tissue,human lung tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-Caveolin-1 antibody (Alexa Fluor® 488) [E249] - Caveolae Marker (ab185043)

    Anti-Caveolin-1 antibody (Alexa Fluor® 647) [E249] - Caveolae Marker (ab193892)

    Anti-Caveolin-1 antibody (HRP) [E249] - Caveolae Marker (ab193893)

性能

应用

Our Abpromise guarantee covers the use of ab32577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 19808975
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 0.01M Sodium Citrate Buffer, pH 6.0.
ICC 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
    • 组织特异性Expressed in muscle and lung, less so in liver, brain and kidney.
    • 疾病相关Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
    • 序列相似性Belongs to the caveolin family.
    • 翻译后修饰The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
    • 细胞定位Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BSCL3 antibody
      • CAV antibody
      • CAV1 antibody
      • CAV1_HUMAN antibody
      • caveolae protein, 22 kD antibody
      • caveolin 1 alpha isoform antibody
      • caveolin 1 beta isoform antibody
      • Caveolin 1 caveolae protein 22kDa antibody
      • Caveolin-1 antibody
      • Caveolin1 antibody
      • cell growth-inhibiting protein 32 antibody
      • CGL3 antibody
      • LCCNS antibody
      • MSTP085 antibody
      • OTTHUMP00000025031 antibody
      • PPH3 antibody
      • VIP 21 antibody
      • VIP21 antibody
      see all

    Anti-Caveolin-1 antibody [E249] - Caveolae Marker 图像

    • Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577) + A431 cell lysate

      Predicted band size : 20 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human urinary bladder tissue, staining Caveolin-1 with ab32577 at 1/250 dilution.

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung tissue, staining Caveolin-1 with ab32577 at 1/250 µg/ml.

    • Immunohistochemical analysis of developing Human lung tissue, staining Caveolin-1 with ab32577 at 1/250 dilution.

    Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)参考文献

    This product has been referenced in:
    • Qayyum T  et al. The interrelationships between Src, Cav-1 and RhoGD12 in transitional cell carcinoma of the bladder. Br J Cancer 106:1187-95 (2012). Read more (PubMed: 22353809) »
    • Kaarteenaho R  et al. Epithelial N-cadherin and nuclear ß-catenin are up-regulated during early development of human lung. BMC Dev Biol 10:113 (2010). IHC-P ; Human . Read more (PubMed: 21080917) »

    See all 4 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Kidney)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
    Permeabilization Yes - Wash Buffer from Dako with Tween
    Specification Kidney
    Fixative Paraformaldehyde
    Username

    Mr. Rudolf Jung

    Verified customer

    提交于 Jun 17 2016

    Thank you for contacting us.

    Regarding antibodies ab79397 and ab32577, they both have been tested in IHC with paraffin embedded sections in the species stated on the datasheet. We don’t have preliminary data using them with frozen secti...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HUVEC)
    Loading amount 100000 cells
    Specification HUVEC
    Treatment various doses of VEGF for 24h
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 May 05 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"