Anti-Caveolin-1抗体- Caveolae Marker (ab18199)

概述

  • 产品名称Anti-Caveolin-1抗体- Caveolae Marker
    参阅全部 Caveolin-1 一抗
  • 描述
    兔多克隆抗体to Caveolin-1 - Caveolae Marker
  • 经测试应用适用于: IP, ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, Pig
    预测可用于: Cow
  • 免疫原

    Synthetic peptide corresponding to Mouse Caveolin-1 aa 1-100 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab18607, ab18607, ab18607, ab18607, ab18607)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Heart (Mouse) Tissue Lysate Heart (Human) Tissue Lysate Heart (Rat) Tissue Lysate

性能

应用

Our Abpromise guarantee covers the use of ab18199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

靶标

  • 功能May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • 组织特异性Expressed in muscle and lung, less so in liver, brain and kidney.
  • 疾病相关Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • 序列相似性Belongs to the caveolin family.
  • 翻译后修饰The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • 细胞定位Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BSCL3 antibody
    • CAV antibody
    • CAV1 antibody
    • CAV1_HUMAN antibody
    • caveolae protein, 22 kD antibody
    • caveolin 1 alpha isoform antibody
    • caveolin 1 beta isoform antibody
    • Caveolin 1 caveolae protein 22kDa antibody
    • Caveolin-1 antibody
    • Caveolin1 antibody
    • cell growth-inhibiting protein 32 antibody
    • CGL3 antibody
    • LCCNS antibody
    • MSTP085 antibody
    • OTTHUMP00000025031 antibody
    • PPH3 antibody
    • VIP 21 antibody
    • VIP21 antibody
    see all

Anti-Caveolin-1 antibody - Caveolae Marker 图像

  • All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/ml

    Lane 1 : Mouse Heart Lysate
    Lane 2 : Human Heart Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • Caveolin 1 - Caveolae Marker was immunoprecipitated using 0.5mg Mouse Heart whole tissue lysate, 5µg of Rabbit polyclonal to Caveolin 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Heart whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab18199.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 20kDa: Caveolin 1 - Caveolae Marker.
  • ICC/IF image of ab18199 stained NIH/3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18199, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • ab18199 staining human HT1080 cells by ICC/IF.  Cells were PFA fixed and permeabilized in Triton X-100 prior to incubating with ab18199 at 10 µg/ml for 1 hour at 20°C.  A Texas Red® conjugated donkey anti-rabbit antibody was used as the secondary.

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  • ICC/IF image of ab18199 stained MEF7cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18199, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)参考文献

This product has been referenced in:
  • Mehta A  et al. Intrinsic hepatocyte dedifferentiation is accompanied by upregulation of mesenchymal markers, protein sialylation and core alpha 1,6 linked fucosylation. Sci Rep 6:27965 (2016). WB ; Rat . Read more (PubMed: 27328854) »
  • Dou X & Charness ME Effect of lipid raft disruption on ethanol inhibition of l1 adhesion. Alcohol Clin Exp Res 38:2707-11 (2014). ICC/IF . Read more (PubMed: 25421507) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Lung)
Loading amount 25 µg
Specification Lung
Gel Running Conditions Reduced Denaturing (15% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
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提交于 Dec 10 2012

Thank you for your reply.

I am sending you 3 new vials of the product and I have set them to ship on Monday for Tuesday delivery. They should be delivered before 10.30am on Tuesday morning. Your new order number is **.

If you experien...

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Thank you for your reply.

Having talked to my colleagues we can go ahead and replace those antibodies for you; however before we do we would like to understand why there was such a delay in the package being delivered to you, so it does not ha...

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Thank you for calling Abcam earlier today.

I am sorry that ab18199 was not working for you. I am going to talk to my colleagues about the best way to proceed win this matter.

I just had a few follow up questions:

1 - Did you ...

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Gracias por contactarnos de nuevo.

El anticuerpo Anti-Caveolin 1 (ab18199) ha sido testado en inmunoprecipitación, Western Blot e immunohistoquímica en secciones embebidas en parafina. Al haberse validado en estas aplicaciones e...

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Gracias por contactarnos.

El producto ab66620 tiene un precio de €380.00 y actualmente en nuestra oficina de Inglaterra queda una unidad es stock. Los productos ab31163 y ab18199 valen €410.00 cada uno y tenemos suficientes unidades...

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Thank you very much for your interest in Abcam antibodies.

For most of the antibodies we have in our catalog it is possible to know the immunogen sequence or at least the immunogenic region and compare it with the sequence of interest.
...

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Thank you for contacting us.

Here is the information I have received from our lab:

We haven’t tested this ab for crossreactivity with caveolin 2 and caveolin 3 but I’ve done a quick blast of our immunogen sequence again...

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Thank you for your inquiry. We do not assign strict expiration dates for our products, however we follow general guidelines: Antibodies stored at 4C may be viable up to 1 year, those stored at -20C may be viable for up to 5 years, and those stored at -...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (OVA lung)
Specification OVA lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Username

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Verified customer

提交于 Jun 06 2011

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