Anti-Caveolin-1抗体(ab39541)

概述

  • 产品名称Anti-Caveolin-1抗体
    参阅全部 Caveolin-1 一抗
  • 描述
    兔多克隆抗体to Caveolin-1
  • 特异性This antibody detects endogenous levels of total Caveolin-1 protein.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to Human Caveolin-1.
    Database link: Q03135

性能

应用

Our Abpromise guarantee covers the use of ab39541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 20 kDa.
ELISA 1/20000.
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • 组织特异性Expressed in muscle and lung, less so in liver, brain and kidney.
  • 疾病相关Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • 序列相似性Belongs to the caveolin family.
  • 翻译后修饰The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • 细胞定位Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BSCL3 antibody
    • CAV antibody
    • CAV1 antibody
    • CAV1_HUMAN antibody
    • caveolae protein, 22 kD antibody
    • caveolin 1 alpha isoform antibody
    • caveolin 1 beta isoform antibody
    • Caveolin 1 caveolae protein 22kDa antibody
    • Caveolin-1 antibody
    • Caveolin1 antibody
    • cell growth-inhibiting protein 32 antibody
    • CGL3 antibody
    • LCCNS antibody
    • MSTP085 antibody
    • OTTHUMP00000025031 antibody
    • PPH3 antibody
    • VIP 21 antibody
    • VIP21 antibody
    see all

Anti-Caveolin-1 antibody 图像



  • Predicted band size : 20 kDa
    Western blot analysis of the extracts from NIH/3T3 cells untreated or treated with H2O2 using Caveolin 1 antibod (ab39541) and Caveolin 1(Phospho-Tyr14) antibody.

    Lane 1: Using ab39541;

    Lane 2: Using ab39541 preincubated with synthesized peptide;

    Lane 3: Using Caveolin 1(Phospho-Tyr14) antibody;

    Lane 4: Using Caveolin 1(Phospho-Tyr14) antibody preincubated with synthesized peptide.

  • ab39541 staining caveolin in human lung.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Caveolin-1 antibody (ab39541)参考文献

ab39541 has not yet been referenced specifically in any publications.

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I am reluctant to recommend this for staining chicken samples, as the human immunogen sequence differs at three amino acids around tyrosine 14 from the chicken sequence:

http://www.uniprot.org/uniprot/...

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