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This antibody can be used as a marker for lipid raft fractions.
Our Abpromise guarantee covers the use of ab2910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500. PubMed: 17502389|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20351069|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human Caveolin-1 peptide (ab4928).|
|IHC-P||Use at an assay dependent concentration.|
Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded human cardiac tissue, labelling Caveolin 1 with ab2910 at a dilution of 1/10000 incubated for 2 hours at 21°C in TBS / BSA / Aside solution. Heat mediated antigen retrival was performed with citric acid. Blocking was with 1% BSA incubated for 10 minutes at 21°C. Secondary used was a goat anti-rabbit polyclonal biotin conjugate at 1/300.Image shows strong immunopositivity at the membranes of cardiomyocytes ( invaginations of immunostaining show points of branching of each myocyte). There is intense positivity in the smooth muscle of of arterioles. Not seen is the intense positivity of what seems to be the endothelial lining cells of the endocardium.
Rat astrocytes stained with fluorescently labelled Caveolin-1 antibody. Primary antibody is ab2910 (dilution 1/500) and the secondary antibody is Texas red labelled anti-rabbit IgG (dilution1/1000).
This image was kindly supplied as part of the review submitted by Donghui Zhu.
ab2910 at 1/250 dilution staining mouse NIH 3T3 cells by Immunocytochemistry (panel B). The antibody was incubated with the paraformaldehyde fixed cells for 12 hours. Bound antibody was detected using an Alexa Fluor ® 594 conjugated Goat anti-rabbit antibody (ab150080). Panel A shows staining with a mouse anti-Caveolin 1 antibody (clone 2297). Panel C shows the merged image.
This image is courtesy of an Abreview by William Ackerman.
ab2910 staining Caveolin-1 - Caveolae Marker in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/200 in PBS + 0.05% Saponin) for 1 hour at 37°C. A Cy3®-conjugated Donkey anti-rabbit polyclonal (1/500) was used as the secondary antibody.
ab2910 diluted 1/500 and was incubated with A549 whole cell lysate and a Protein A/G matrix for 16 hours at 4°C to achieve immunoprecipitation. 400 µg of lysate was present in the input.
An HRP-conjugated goat anti-rabbit was used for the Western Blot step.
Lane 1: Whole Cell
Lane 2: IP-cav-1
Lane 3: Unrelated antibody
Lane 4: Unrelated antibody
Western blot of caveolin-1 on rat heart protein extract using ab2910.