Anti-Caveolin-1抗体[7C8] (ab17052)

概述

  • 产品名称
    Anti-Caveolin-1抗体[7C8]
    参阅全部 Caveolin-1 一抗
  • 描述
    小鼠单克隆抗体[7C8] to Caveolin-1
  • 特异性
    The monoclonal antibody 7C8 recognizes caveolin-1a as well as caveolin-1ß, which are present in many tissues, like aorta, heart, muscle, lung, adipose white, brown and epidydimal fat.
  • 经测试应用
    适用于: ICC/IF, Flow Cyt, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Human, Pig
  • 免疫原

    Tissue, cells or virus corresponding to Rat Caveolin-1. Immunogen: GLUT4-containing vesicles immunoadsorbed from low density microsomes of rat adipocytes (Sprague Dawley). The antibody recognises epitope between residue 32 and the C-terminus.

  • 阳性对照
    • Adipocytes (3T3-L1 adipocytes)

性能

应用

Our Abpromise guarantee covers the use of ab17052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. Used at a dilution of 1/500 for 2 hrs on hamster CHO cells
Flow Cyt Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. Detects a band of approximately 21, 19 kDa.
IP Use at an assay dependent concentration.

靶标

  • 功能
    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • 组织特异性
    Expressed in muscle and lung, less so in liver, brain and kidney.
  • 疾病相关
    Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • 序列相似性
    Belongs to the caveolin family.
  • 翻译后修饰
    The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • 细胞定位
    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BSCL3 antibody
    • CAV antibody
    • CAV1 antibody
    • CAV1_HUMAN antibody
    • caveolae protein, 22 kD antibody
    • caveolin 1 alpha isoform antibody
    • caveolin 1 beta isoform antibody
    • Caveolin 1 caveolae protein 22kDa antibody
    • Caveolin-1 antibody
    • Caveolin1 antibody
    • cell growth-inhibiting protein 32 antibody
    • CGL3 antibody
    • LCCNS antibody
    • MSTP085 antibody
    • OTTHUMP00000025031 antibody
    • PPH3 antibody
    • VIP 21 antibody
    • VIP21 antibody
    see all

图片

  •  ab17052 staining Caveolin-1 in human Hacat keratinocyte cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 1% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/75 for 24 hours at 4°C. The secondary antibody used was conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.

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  • Overlay histogram showing CHO cells stained with ab17052 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17052, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in CHO cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • Immunocytochemical analysis of Mouse Bend.3 cells, labeling Caveolin-1 with ab17052. Cells were paraformaldehyde fixed, permeabilized with PBS + 0.1% Triton (PBT), and blocked with 10% serum for 1 hour at 22°C. Staining with ab17052 (diluted 1/200) was for 16 hours at 4°C.

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  • Anti-Caveolin-1 antibody [7C8] (ab17052) at 1/500 dilution + HUVEC whole cell lysate at 20 µg
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview.

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  • ab17052 at a 1/500 dilution staining hamster CHO cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 10% serum prior to incubation with the antibody. Bound antibody was detected using a FITC conjugated goat anti-mouse antibody.

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文献

This product has been referenced in:
  • Luo X  et al. Caveolin 1-related autophagy initiated by aldosterone-induced oxidation promotes liver sinusoidal endothelial cells defenestration. Redox Biol 13:508-521 (2017). Read more (PubMed: 28734243) »
  • Sultana A  et al. Inhibition of inflammatory signaling pathways in 3T3-L1 adipocytes by apolipoprotein A-I. FASEB J 30:2324-35 (2016). Read more (PubMed: 26965683) »

See all 14 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Cardiac myocyte)
Permeabilization
Yes - Triton 0.1%
Specification
Cardiac myocyte
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 31 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Bos taurus Cell lysate - other (HEK293T cells and BoMAC (bovine macrophages) Lyse)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
HEK293T cells and BoMAC (bovine macrophages) Lyse
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Mar 08 2016

Application
Western blot
Sample
Rat Cell lysate - whole cell (pericytes)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
pericytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 19 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (pericytes)
Permeabilization
Yes - 0.1% Triton in PBS
Specification
pericytes
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 18 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HUVEC)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
HUVEC
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Dec 07 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HRMEC)
Permeabilization
Yes - 0.1% triton in PBS
Specification
HRMEC
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 23 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (PAEC)
Specification
PAEC
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 27 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Mouse Cell (bEnd.3)
Specification
bEnd.3
Permeabilization
Yes - PBT (PBS+0.1%Triton)
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 17 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you very much for your call today and for letting us know about the trouble with ab17052.

Please keep me updated about the results after trying the protocol alterations that we discussed. If the antibody still does not show the correct ...

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