This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Cav2.3抗体(ab63705)

概述

  • 产品名称Anti-Cav2.3抗体
  • 描述
    兔多克隆抗体to Cav2.3

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • 种属反应性

    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 2200 to the C-terminus of Rat Cav2.3.

    (Peptide available as ab66709.)

性能

应用

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
ELISA Use at an assay dependent dilution. This antibody gave a positive result in ELISA against the immunizing peptide (ab66709).

靶标

  • 功能Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1E gives rise to R-type calcium currents. R-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by nickel, and partially by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), omega-conotoxin-GVIA (omega-CTx-GVIA), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing alpha-1E subunit could be involved in the modulation of firing patterns of neurons which is important for information processing.
  • 组织特异性Expressed in neuronal tissues and in kidney.
  • 序列相似性Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1E subfamily.
    Contains 1 EF-hand domain.
  • 结构域Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • alpha-1 polypeptide antibody
    • BII antibody
    • Brain calcium channel II antibody
    • CAC 1E antibody
    • CAC1E antibody
    • CAC1E_HUMAN antibody
    • CACH 6 antibody
    • CACH6 antibody
    • CACNA1E antibody
    • Cacnl1 a6 antibody
    • CACNL1A6 antibody
    • Calcium channel antibody
    • Calcium channel, L type, alpha 1 polypeptide, isoform 6 antibody
    • calcium channel, R type antibody
    • Cav 2.3 antibody
    • Cchra 1 antibody
    • Cchra1 antibody
    • isoform 6 antibody
    • L type antibody
    • Voltage dependent R type calcium channel subunit alpha 1E antibody
    • Voltage gated calcium channel subunit alpha Cav2.3 antibody
    • Voltage-dependent R-type calcium channel subunit alpha-1E antibody
    • Voltage-gated calcium channel subunit alpha Cav2.3 antibody
    see all

Anti-Cav2.3 antibody 图像

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • ICC/IF image of ab63705 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63705, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) PC12 cells at 1µg/ml.

Anti-Cav2.3 antibody (ab63705)参考文献

ab63705 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

提交于 Sep 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"