Anti-CaV1.3抗体[S38-8] (ab84811)

概述

  • 产品名称Anti-CaV1.3抗体[S38-8]
    参阅全部 CaV1.3 一抗
  • 描述
    小鼠单克隆抗体[S38-8] to CaV1.3
  • 特异性No cross-reactivity against Cav1.2
  • 经测试应用适用于: IP, ICC/IF, WB, Agglutinationmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
  • 免疫原

    Fusion protein corresponding to C terminal amino acids 2025-2161 of Rat Cav1.3

  • 阳性对照
    • Rat brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab84811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 - 10 µg/ml.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 245 kDa.
Agglutination Use 1-2µg for 106 cells.

靶标

  • 功能Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).
  • 组织特异性Expressed in pancreatic islets and in brain, where it has been seen in cerebral cortex, hippocampus, basal ganglia, habenula and thalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in skeletal muscle.
  • 序列相似性Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1D subfamily.
  • 结构域Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • alpha-1 polypeptide antibody
    • CAC1D_HUMAN antibody
    • CACH3 antibody
    • CACN4 antibody
    • CACNA 1D antibody
    • Cacna1d antibody
    • CACNL1A2 antibody
    • Calcium channel antibody
    • Calcium channel L type alpha 1 polypeptide isoform 2 antibody
    • Calcium channel neuroendocrine/brain type alpha 1 subunit antibody
    • Calcium channel voltage dependent L type alpha 1 antibody
    • Calcium channel voltage dependent L type alpha 1D subunit antibody
    • Cav1.3 antibody
    • CCHL1A2 antibody
    • isoform 2 antibody
    • L type antibody
    • PASNA antibody
    • SANDD antibody
    • Voltage dependent L type calcium channel subunit alpha 1D antibody
    • Voltage gated calcium channel alpha 1 subunit antibody
    • Voltage gated calcium channel alpha subunit Cav1.3 antibody
    • Voltage gated calcium channel subunit alpha Cav1.3 antibody
    • Voltage-dependent L-type calcium channel subunit alpha-1D antibody
    • Voltage-gated calcium channel subunit alpha Cav1.3 antibody
    see all

Anti-CaV1.3 antibody [S38-8] 图像

  • ab84811 staining CaV1.3 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
  • All lanes : Anti-CaV1.3 antibody [S38-8] (ab84811) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Rat tissue lysate


    Predicted band size : 245 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab84811 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84811, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-CaV1.3 antibody [S38-8] (ab84811)参考文献

ab84811 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (atrium)
Specification atrium
Fixative Paraformaldehyde
Permeabilization Yes - triton-X 100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
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Verified customer

提交于 Apr 25 2012

Application Immunohistochemistry (Frozen sections)
Sample Rabbit Tissue sections (heart)
Specification heart
Fixative Paraformaldehyde
Permeabilization Yes - triton-X 100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Feb 18 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (heart)
Specification heart
Fixative Paraformaldehyde
Permeabilization Yes - triton-X 100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 23 2012

Application Immunocytochemistry
Sample Rabbit Cell (atrial cell)
Specification atrial cell
Fixative Paraformaldehyde
Permeabilization Yes - triton-X
Username

Abcam user community

Verified customer

提交于 Jan 20 2012

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