Anti-CaV1.3抗体[N38/8] (ab84811)

概述

  • 产品名称
    Anti-CaV1.3抗体[N38/8]
    参阅全部 CaV1.3 一抗
  • 描述
    小鼠单克隆抗体[N38/8] to CaV1.3
  • 特异性
    No cross-reactivity against Cav1.2
  • 经测试应用
    适用于: IP, ICC/IF, WB, Agglutinationmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
  • 免疫原

    Fusion protein corresponding to C terminal amino acids 2025-2161 of Rat Cav1.3

  • 阳性对照
    • Rat brain lysate
  • 常规说明

    At Abcam we are committed to ensuring that our customers have access to the complete dataset available for our products to allow you to select the best product for your research needs. With this in mind, for this product we highlight the fact that it is known by two different clone numbers. It's primary clone number is N38/8, but you may also see it referred to as S38-8 in literature.

    The clone number has been updated from S38-8 to N38/8, both clone numbers name the same antibody clone.

     

性能

应用

Our Abpromise guarantee covers the use of ab84811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 - 10 µg/ml.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 245 kDa.
Agglutination Use 1-2µg for 106 cells.

靶标

  • 功能
    Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).
  • 组织特异性
    Expressed in pancreatic islets and in brain, where it has been seen in cerebral cortex, hippocampus, basal ganglia, habenula and thalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in skeletal muscle.
  • 序列相似性
    Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1D subfamily.
  • 结构域
    Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • alpha-1 polypeptide antibody
    • CAC1D_HUMAN antibody
    • CACH3 antibody
    • CACN4 antibody
    • CACNA 1D antibody
    • Cacna1d antibody
    • CACNL1A2 antibody
    • Calcium channel antibody
    • Calcium channel L type alpha 1 polypeptide isoform 2 antibody
    • Calcium channel neuroendocrine/brain type alpha 1 subunit antibody
    • Calcium channel voltage dependent L type alpha 1 antibody
    • Calcium channel voltage dependent L type alpha 1D subunit antibody
    • Cav1.3 antibody
    • CCHL1A2 antibody
    • isoform 2 antibody
    • L type antibody
    • PASNA antibody
    • SANDD antibody
    • Voltage dependent L type calcium channel subunit alpha 1D antibody
    • Voltage gated calcium channel alpha 1 subunit antibody
    • Voltage gated calcium channel alpha subunit Cav1.3 antibody
    • Voltage gated calcium channel subunit alpha Cav1.3 antibody
    • Voltage-dependent L-type calcium channel subunit alpha-1D antibody
    • Voltage-gated calcium channel subunit alpha Cav1.3 antibody
    see all

图片

  • ab84811 staining CaV1.3 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
  • All lanes : Anti-CaV1.3 antibody [N38/8] (ab84811) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Rat tissue lysate


    Predicted band size : 245 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab84811 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84811, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

文献

ab84811 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (atrium)
Specification
atrium
Fixative
Paraformaldehyde
Permeabilization
Yes - triton-X 100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 25 2012

Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (heart)
Specification
heart
Fixative
Paraformaldehyde
Permeabilization
Yes - triton-X 100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 18 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Specification
heart
Fixative
Paraformaldehyde
Permeabilization
Yes - triton-X 100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 23 2012

Application
Immunocytochemistry
Sample
Rabbit Cell (atrial cell)
Specification
atrial cell
Fixative
Paraformaldehyde
Permeabilization
Yes - triton-X
Username

Abcam user community

Verified customer

提交于 Jan 20 2012

I am very pleased to hear you would like to accept our offer and test ab84811 in rabbit. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rabbit and include this code in th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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