Anti-CatSper2抗体(ab66642)

概述

  • 产品名称Anti-CatSper2抗体
    参阅全部 CatSper2 一抗
  • 描述
    兔多克隆抗体to CatSper2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: MEMDQDDRVW PRDSLFRYFE LLEKLQYNLE ERKKLQEFAV QALMNLEDK, corresponding to C terminal amino acids 482-530 of Human CatSper2

  • 阳性对照
    • HepG2 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab66642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 62 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性CatSper2 belongs to a family of putative cation channels that are specific to spermatozoa and localize to the flagellum. Calcium ions play a primary role in the regulation of sperm motility. The protein family features a single repeat with six membrane spanning segments and a predicted calcium selective pore region. A second, closely linked copy of the gene has been identified. Although multiple transcript variants encoding protein isoforms have been characterized, they seem to be only transcribed from this gene. Additional splice variants have been described but their full length nature has not been determined.
  • 细胞定位Cell projection, cilium, flagellum; Multi-pass membrane protein
  • 数据库链接
  • 别名
    • Cation channel sperm associated 2 antibody
    • Cation channel sperm associated protein 2 antibody
    • Catsper 2 antibody
    • MGC33346 antibody
    • Putative ion channel protein CATSPER2 variant 1 antibody
    • Putative ion channel protein CATSPER2 variant 2 antibody
    • Putative ion channel protein CATSPER2 variant 3 antibody
    • Sperm associated cation channel 2 antibody
    • Sperm ion channel antibody
    see all

Anti-CatSper2 antibody 图像

  • All lanes : Anti-CatSper2 antibody (ab66642) at 1.25 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 62 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.

Anti-CatSper2 antibody (ab66642)参考文献

ab66642 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

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Yes of course. We are also awaiting the reply from our supplier and will follow up with them early next month if we do not hear from them sooner. We will forward you any information regarding the immunogen sequence as soon as we get it.

Thanks for letting me know you have already received a replacement. Best of luck in your research.

So glad to hear you liked the brownies!

When you have a chance, please let me knowif you would like thefree replacement antibody and I'll be happy to send it along.

Havea great weekend!



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