兔多克隆抗体to Cathepsin V
ab124656 detects endogenous levels of fragment of activated CATL2 resulting from cleavage adjacent to Leu114.
A synthetic peptide, corresponding to internal sequence amino acids of Human Cathepsin V.
Human breast tissue; 293 and COS cell extracts, treated with etoposide 25uM 1h.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium azide Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride Note: PBS (without Mg 2+, Ca 2+)
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 37 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Cysteine protease. May have an important role in corneal physiology.
Predominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin.
Belongs to the peptidase C1 family.
Information by UniProt
CAT L2 antibody
Cathepsin L2 antibody
Cathepsin U antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin V antibody (ab124656)
ab124656, at 1/500 dilution, staining Cathepsin V in Formalin-Fixed, Paraffin-Embedded Human breast tissue by Immunohistochemistry.
Western blot - Anti-Cathepsin V antibody (ab124656)
All lanes : Anti-Cathepsin V antibody (ab124656) at 1/500 dilution Lane 1 : 293 cell extracts, treated with etoposide 25uM 1h Lane 2 : COS cell extracts, treated with etoposide 25uM 1h Lane 3 : 293 cell extracts, treated with etoposide 25uM 1h with synthesized peptide Predicted band size : 37 kDa
has not yet been referenced specifically in any publications.
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