概述

  • 产品名称Anti-Cathepsin S抗体
    参阅全部 Cathepsin S 一抗
  • 描述
    山羊多克隆抗体to Cathepsin S
  • 特异性This antibody is specific to Cathepsin S.
  • 经测试应用适用于: IHC-P, IP, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
  • 免疫原

    Synthetic peptide mapping to the C terminus of human Cathepsin S. (Peptide available as ab54080.)

  • 阳性对照
    • IHC-P: human lymphnod

性能

应用

Our Abpromise guarantee covers the use of ab18822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 20 µg/ml.
IP Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 38 kDa).Can be blocked with Cathepsin S peptide (ab54080). One customer reports that overnight incubation with this antibody at room temperature gave the best staining in Western blot.
IHC-Fr Use a concentration of 20 µg/ml.

靶标

Anti-Cathepsin S antibody 图像

  • IHC image of ab18822 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18822, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Cathepsin S antibody (ab18822)参考文献

This product has been referenced in:
  • Huyard F  et al. Remodeling of aorta extracellular matrix as a result of transient high oxygen exposure in newborn rats: implication for arterial rigidity and hypertension risk. PLoS One 9:e92287 (2014). IF ; Rat . Read more (PubMed: 24743169) »
  • Lohoefer F  et al. Quantitative expression and localization of cysteine and aspartic proteases in human abdominal aortic aneurysms. Exp Mol Med 46:e95 (2014). WB, IP ; Human . Read more (PubMed: 24833013) »

See all 5 Publications for this product

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (Primary microglia and neurons)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 5 µg
Specification Primary microglia and neurons
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Nov 29 2016

Spleen is a good choice. For examples of blots of spleen stained for cathepsin S, see the datasheets for antibodies ab72954 and ab134157.

If you do not have spleen lysate, we have ab29393.

Thank you for your inquiry.

I can confirm that the immunogen shows a high homology with pig and ab18822 was successfully used in a publication with porcine protein:

Cabrera JA et al. Altered expression of mitochondrial electron tran...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MDA MB 468)
Loading amount 10 µg
Specification MDA MB 468
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 May 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount 10 µg
Specification NIH 3T3 cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 May 14 2009

I was informed today that the positive control is Human Lymphnod tissue section. Please do not hesitate to contact us again if you need more information,

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Ventral mouse prostate and tumor tissue from mouse)
Loading amount 50 µg
Specification Ventral mouse prostate and tumor tissue from mouse
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2 %
Username

Mrs. Åsa Skytt

Verified customer

提交于 Jun 19 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"