Anti-Cathepsin S抗体(ab103848)

概述

  • 产品名称
    Anti-Cathepsin S抗体
    参阅全部 Cathepsin S 一抗
  • 描述
    兔多克隆抗体to Cathepsin S
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Cathepsin S.

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Lung (Human) Tissue Lysate; Spleen (Human) Tissue Lysate - adult normal tissue;

性能

应用

Our Abpromise guarantee covers the use of ab103848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 mg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 37 kDa).

靶标

  • 功能
    Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.
  • 序列相似性
    Belongs to the peptidase C1 family.
  • 细胞定位
    Lysosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cathepsin S antibody
    • CathepsinS antibody
    • CATS_HUMAN antibody
    • Ctss antibody

图片

  • All lanes : Anti-Cathepsin S antibody (ab103848) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 25 kDa (why is the actual band size different from the predicted?)


    Exposure time: 20 minutes


    We hypothesize that the 25 kDa band represents the mature form of Cathepsin S. Abcam welcomes customer feedback.

实验方案

文献

ab103848 has not yet been referenced specifically in any publications.

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