Western blot - Anti-Cathepsin L antibody (ab95154)
All lanes : Anti-Cathepsin L antibody (ab95154) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa Observed band size: 37 kDa Additional bands at: 25 kDa (possible cleavage fragment), 35 kDa (possible cleavage fragment), 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The bands observed at 35 + 25 kDa could potentially be cleaved forms of Cathepsin L due to the presence of both a 17 amino acid signal peptide and a 96 amino acid activation peptide. This suggests that both the pre and mature form are observed in this blot.