Anti-Cathepsin K抗体(ab85716)

概述

  • 产品名称Anti-Cathepsin K抗体
    参阅全部 Cathepsin K 一抗
  • 描述
    兔多克隆抗体to Cathepsin K
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog, Pig, Non Human Primates
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Cathepsin K.

    (Peptide available as ab97980.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Human Bone Marrow Tissue; Human Lung Tissue; U2OS Whole Cell; MCF7 Whole Cell; MDA-MB-231 Whole Cell; MDA-MB-361 Whole Cell; Saos 2 Whole Cell.

性能

应用

Our Abpromise guarantee covers the use of ab85716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 37 kDa).

靶标

Anti-Cathepsin K antibody 图像

  • All lanes : Anti-Cathepsin K antibody (ab85716) at 1 µg/ml

    Lane 1 : Bone marrow (Human) Tissue Lysate
    Lane 2 : Lung (Human) Tissue Lysate
    Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

Anti-Cathepsin K antibody (ab85716)参考文献

ab85716 has not yet been referenced specifically in any publications.

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