Anti-Cathepsin K抗体(ab66237)

概述

  • 产品名称Anti-Cathepsin K抗体
    参阅全部 Cathepsin K 一抗
  • 描述
    小鼠单克隆抗体to Cathepsin K
  • 经测试应用适用于: ELISA, WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus Monkey, Rhesus monkey, Opossum
  • 免疫原

    Recombinant fragment with tag: KCRGYREIPE GNEKALKRAV ARVGPVSVAI DASLTSFQFY SKGVYYDESC NSDNLNHAVL AVGYGIQKGN KHWIIKNSWG ENWGNKGYIL MARNKNNACG IANLASFPKM, corresponding to amino acids 220-331 of Human Cathepsin K

  • 阳性对照
    • Cathepsin K transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab66237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 10 µg/ml.

靶标

Anti-Cathepsin K antibody 图像

  • All lanes : Anti-Cathepsin K antibody (ab66237) at 1 µg/ml

    Lane 1 : Cathepsin K transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
  • ab66237 at 10 ug/ml staining Cathepsin K in human kidney tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • ICC/IF image of ab66237 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66237, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing U20S cells stained with ab66237 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66237, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Cathepsin K antibody (ab66237)参考文献

ab66237 has not yet been referenced specifically in any publications.

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