概述

  • 产品名称
    Anti-Cathepsin K抗体
    参阅全部 Cathepsin K 一抗
  • 描述
    兔多克隆抗体to Cathepsin K
  • 特异性
    ab19027 recognises cathepsin K.
  • 经测试应用
    适用于: IHC-Fr, ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Zebrafish
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 300-329 of Rat Cathepsin K which includes residue 321. (Peptide available as ab41720.)

性能

应用

Our Abpromise guarantee covers the use of ab19027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 40, 29 kDa (predicted molecular weight: 37 kDa).Can be blocked with Cathepsin K peptide (ab41720).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
  • 组织特异性
    Predominantly expressed in osteclasts (bones).
  • 疾病相关
    Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.
  • 序列相似性
    Belongs to the peptidase C1 family.
  • 细胞定位
    Lysosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cathepsin K antibody
    • Cathepsin O antibody
    • Cathepsin O1 antibody
    • Cathepsin O2 antibody
    • Cathepsin X antibody
    • CATK_HUMAN antibody
    • CTS02 antibody
    • Ctsk antibody
    • CTSO antibody
    • CTSO1 antibody
    • CTSO2 antibody
    • MGC23107 antibody
    • PKND antibody
    • PYCD antibody
    see all

图片

  • ab19027 staining Cathepsin K in mouse bone tissue section, in which human MDA-MB-231 Breast Cancer cells were grown, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation and then blocking with 10% Casein for 30 minutes at 22°C was performed. The primary antibody was diluted 1/200 and incubated with sample in 10% Casein for 17 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution.

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  • ab19027 staining Cathepsin K in the Human cell line HepaRG by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 0.1% and blocked with 1% milk for 30 minutes at 21°C. Samples were incubated with primary antibody (1ug/ml in 1% milk) for 30 minutes. An Alexa Fluor®594-conjugated Goat anti-rabbit IgG polyclonal(1/400) was used as the secondary antibody.

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  • ab19027 staining Cathepsin K in rat osteoclasts by Immunohistochemistry (Frozen sections). 21 day old rat bone was fixed in methanol and then blocked using 20% serum for 30 minutes at 22°C. Samples were then incubated with primary antibody at 1/50 for 3 hours at 22°C. The secondary antibody used was an anti-rabbit IgG conjugated to Cy3® (red) used at a 1/1000 dilution.
  • ab19027 staining Cathepsin K in cultured human osteoclasts by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100 PBS, blocked with 1% BSA for 1 hour at 25°C and then incubated with ab19027 at 2µg/ml for 30 minutes at 25°C. The secondary used was a FITC conjugated goat anti-rabbit polyclonal used at a 1/00 dilution.

    Red = Actin
    Blue = DAPI
    Green / Yellow = Cathepsin K

    See Abreview

文献

This product has been referenced in:
  • Lin YY  et al. Excavatolide B Attenuates Rheumatoid Arthritis through the Inhibition of Osteoclastogenesis. Mar Drugs 15:N/A (2017). IHC-P ; Rat . Read more (PubMed: 28067799) »
  • Jiang T  et al. Distribution of Cathepsin K in Late Stage of Tooth Germ Development and Its Function in Degrading Enamel Matrix Proteins in Mouse. PLoS One 12:e0169857 (2017). Read more (PubMed: 28095448) »

See all 34 Publications for this product

客户评价及客户问答

Merci de nous avoir contactés et de nous avoir signalé le problème avec le ab19027. Les détails fournis sur le protocole utilisé vont nous permettre de prendre les dispositions nécessaires pour assurer une bonne qualité de nos produits. Comme conven...

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Axolotl Cell (Osteoclasts in limb section)
Permeabilization
Yes - Triton X-100 0.1%
Specification
Osteoclasts in limb section
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
MEMFa
Username

Mr. Camilo Riquelme Guzman

Verified customer

提交于 Sep 06 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - Triton X-100
Specification
Bone
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Dr. Tina Yuan

Verified customer

提交于 Apr 24 2017

Application
ELISA
Sample
Mouse Recombinant protein (Recombinant, active mouse CTSK (Biovision 7597))
Specification
Recombinant, active mouse CTSK (Biovision 7597)
Blocking step
BSA as blocking agent for 2 hour(s) and 21 minute(s) · Concentration: 1% · Temperature: 25°C
Type
Indirect
Username

Abcam user community

Verified customer

提交于 Feb 01 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Vascular smooth muscle cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Vascular smooth muscle cells
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Dec 02 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse Calvarial)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
25 µg
Treatment
Insulin as positive control
Specification
Mouse Calvarial
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Abcam user community

Verified customer

提交于 Aug 13 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Mouse Cell (hepatocytes)
Specification
hepatocytes
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 17 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
PBS 1% BSA + 5% NGS as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Sample
Mouse Tissue sections (bone)
Specification
bone
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 24 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepaRG)
Specification
HepaRG
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mrs. Alina Macovei

Verified customer

提交于 Feb 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepaRG)
Loading amount
25 µg
Specification
HepaRG
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Mrs. Alina Macovei

Verified customer

提交于 Jun 19 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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