Anti-Cathepsin K抗体[3F9] (ab37259)

概述

  • 产品名称Anti-Cathepsin K抗体[3F9]
    参阅全部 Cathepsin K 一抗
  • 描述
    小鼠单克隆抗体[3F9] to Cathepsin K
  • 经测试应用适用于: ELISA, WB, IP, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fusion protein, corresponding to amino acids 115-329 of Human Cathepsin K. The protein contains at the N-terninal end a His tag (6x) and 6 additional amino acid residues (MRGSHHHHHHGS).

  • 表位The monoclonal antibody ab37259 (clone 3F9) reacts with an epitope located between aa 115-329: APDSVDYRKKGYVTPNQGQCGSCWAFSSVGALEGQLKKKTGKLLNLSPQNLVDCVSENDGC GGGYMTNAFQYVQKNRGIDSEDAYPYVGQEESCMYNPTGKAAKCRGYREIPEGNEKALKRA VARVGPVSVAIDASLTSFQFSKGVYYDESCNSDNLNHAVLAVGYGIQKGNKHWIIKNSWGE NWGNKGYILMARNKNNACGIANLASFPKM

性能

应用

Our Abpromise guarantee covers the use of ab37259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37, 40 kDa (predicted molecular weight: 37 kDa).
IP Use at an assay dependent concentration. PubMed: 24833013
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
  • 组织特异性Predominantly expressed in osteclasts (bones).
  • 疾病相关Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.
  • 序列相似性Belongs to the peptidase C1 family.
  • 细胞定位Lysosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cathepsin K antibody
    • Cathepsin O antibody
    • Cathepsin O1 antibody
    • Cathepsin O2 antibody
    • Cathepsin X antibody
    • CATK_HUMAN antibody
    • CTS02 antibody
    • Ctsk antibody
    • CTSO antibody
    • CTSO1 antibody
    • CTSO2 antibody
    • MGC23107 antibody
    • PKND antibody
    • PYCD antibody
    see all

Anti-Cathepsin K antibody [3F9] 图像

  • Bone from mouse foot (bone marrow with osteoclasts (positive). IHC-P image was obtained after deparaffinization, antigen retrieval with 0.1 M EDTA (6-8 min, 72 C), drying of slide before staining (10 min, 62 C), and subsequent staining with primary antibody (1/100) in 1% horse serum (1 h, 37 C).
  • Anti-Cathepsin K antibody [3F9] (ab37259) at 1 µg/ml + Human bone tumor tissue lysate - total protein (ab29359) at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 + 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa (possible cleavage fragment).

    Exposure time : 1 minute
  • Overlay histogram showing U20S cells stained with ab37259 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37259, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in U20S cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-Cathepsin K antibody [3F9] (ab37259)参考文献

This product has been referenced in:
  • Foix MP  et al. Morphological, immunohistochemical, and chromosomal analysis of multicystic chromophobe renal cell carcinoma, an architecturally unusual challenging variant. Virchows Arch 469:669-678 (2016). Read more (PubMed: 27631338) »
  • Rao Q  et al. Cathepsin K in the immunohistochemical diagnosis of melanocytic lesions. Int J Clin Exp Pathol 7:1132-9 (2014). IHC-P ; Human . Read more (PubMed: 24696729) »

See all 11 Publications for this product

Product Wall

Grazie per la sua domanda. Vi rispondo in Inglese - se ha bisogno di una traduzione, mi faccia sapere per favore. Grazie!
Thank you for your inquiry.
The protocol on the datasheet is this protocol is from a customer who tested our antibody an...

Read More

Thank you for contacting us and your interest in our products.

I am sorry if the product datasheet has been misleading for your customer. The details provided with the image are to illustrate how this particular IHC staining experiment was pe...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"