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Cathepsin K Activity Assay Kit (Fluorometric) (ab65303) is a fluorescence-based assay that utilizes the preferred cathepsin K substrate sequence LR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin K will cleave the synthetic substrate LR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.
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Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
|CK Cell Lysis Buffer||WM||1 x 25ml|
|CK Inhibitor (1mM)||Red||1 x 1µl|
|CK Reaction Buffer||NM||1 x 5ml|
|CK Substrate Ac-LR-AFC (10mM)||Amber||1 x 200µl|
Our Abpromise guarantee covers the use of ab65303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Cathepsin K Activity measured in tissue lysates (each with protein concentration of 4 mg/mL) after 1 hour of incubation with and without inhibitor (I).
Cathepsin K Activity measured in cell lysates (each with cells concentration of 4e7 cells/mL) after 1 hour of incubation with and without inhibitor (I).
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