小鼠单克隆抗体[AF14D7] to Cathepsin H
Recombinant human Cathepsin H with His tag purified from E.coli
- K562, HepG2, HT29 and C6 cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
Concentration information loading...
Ammonium Sulphate Precipitation
Purified from TCS
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 37 kDa).
Important for the overall degradation of proteins in lysosomes.
Belongs to the peptidase C1 family.
Information by UniProt
- ACC 4 antibody
- ACC 5 antibody
- ACC4 antibody
Western blot - Anti-Cathepsin H antibody [AF14D7] (ab88960)
All lanes :
Anti-Cathepsin H antibody [AF14D7] (ab88960) at 1/1000 dilutionLane 1 :
K562 cell lysateLane 2 :
HepG2 cell lysateLane 3 :
HT29 cell lysateLane 4 :
C6 cell lysatePredicted band size:
37 kDaObserved band size:
25 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
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