概述

  • 产品名称Anti-Cathepsin E抗体
    参阅全部 Cathepsin E 一抗
  • 描述
    兔多克隆抗体to Cathepsin E
  • 特异性This antibody is specific for Cathepsin E.
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide made to the C-terminus of mouse Cathepsin E.

  • 阳性对照
    • Murine reticulocyte lysate. Cathepsin E is expressed abundantly in the stomach, Clara cells and alveolar macrophages of the lung, brain microglia, spleen, and activated B lymphocytes. It is not expressed in resting B lymphocytes.

性能

应用

Our Abpromise guarantee covers the use of ab36996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 42 kDa.
ICC/IF 1/200.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.
  • 组织特异性Expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B-lymphocytes.
  • 序列相似性Belongs to the peptidase A1 family.
  • 翻译后修饰Glycosylated. The nature of the carbohydrate chain varies between cell types. In fibroblasts, the proenzyme contains a high mannose-type oligosaccharide, while the mature enzyme contains a complex-type oligosaccharide. In erythrocyte membranes, both the proenzyme and mature enzyme contain a complex-type oligosaccharide.
    Two forms are produced by autocatalytic cleavage, form I begins at Ile-54, form II begins at Thr-57.
  • 细胞定位Endosome. The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CATE antibody
    • CATE_HUMAN antibody
    • Cathepsin E antibody
    • Cathepsin E form II antibody
    • CTSE antibody
    • Erythrocyte membrane aspartic proteinase antibody
    • Slow moving proteinase antibody
    see all

Anti-Cathepsin E antibody 图像

  • Anti-Cathepsin E antibody (ab36996) at 1/1000 dilution + Murine reticulocyte lysate at 10 µg

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ab36996 staining Cathepsin E at a dilution of 1/2000 in Human stomach and tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The sections were PFA-fixed and subjected to Heat-mediated antigen retrieval in TRIS EDTA Buffer pH 9.0 and permeabilization with DAKO wash buffer with Tween. The primary antibody was diluted 1/2000 in DAKO blocking dilution buffer and incubated with the sample for 30 minutes at 20°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody.

    See Abreview

Anti-Cathepsin E antibody (ab36996)参考文献

This product has been referenced in:
  • Li XL  et al. An essential role for the antiviral endoribonuclease, RNase-L, in antibacterial immunity. Proc Natl Acad Sci U S A 105:20816-21 (2008). ICC/IF ; Mouse . Read more (PubMed: 19075243) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Stomach and Tonsil)
Specification Stomach and Tonsil
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS EDTA Buffer pH 9.0
Permeabilization Yes - Wash Buffer from DAKO with tween
Username

Mr. Rudolf Jung

Verified customer

提交于 May 04 2011

Application Western blot
Sample Mouse Tissue lysate - whole (Pancreas)
Loading amount 20 µg
Specification Pancreas
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"