The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ICC: 1/100 - 1/250.
IHC-P: 1/500. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
WB: 1/1000 - 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Is unsuitable for Flow Cyt or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
组织特异性Expressed in the aorta extrcellular space (at protein level).
疾病相关Ceroid lipofuscinosis, neuronal, 10
序列相似性Belongs to the peptidase A1 family. Contains 1 peptidase A1 domain.
翻译后修饰N- and O-glycosylated.
细胞定位Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).