RabMAb

Anti-Cathepsin D抗体[EPR3056Y] (ab75811)

概述

  • 产品名称Anti-Cathepsin D抗体[EPR3056Y]
    参阅全部 Cathepsin D 一抗
  • 描述
    兔单克隆抗体[EPR3056Y] to Cathepsin D
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human Cathepsin D

  • 阳性对照
    • MCF-7 and SKBR3 cell lysates. Human liver tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab75811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
ICC
  • 应用说明ICC: 1/100 - 1/250.
    IHC-P: 1/500. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
    WB: 1/1000 - 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

    Is unsuitable for Flow Cyt or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
    • 组织特异性Expressed in the aorta extrcellular space (at protein level).
    • 疾病相关Ceroid lipofuscinosis, neuronal, 10
    • 序列相似性Belongs to the peptidase A1 family.
      Contains 1 peptidase A1 domain.
    • 翻译后修饰N- and O-glycosylated.
    • 细胞定位Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
    • Information by UniProt
    • 数据库链接
    • 别名
      • CatD antibody
      • CATD_HUMAN antibody
      • Cathepsin D heavy chain antibody
      • CD antibody
      • Ceroid lipofuscinosis neuronal 10 antibody
      • CLN10 antibody
      • CPSD antibody
      • ctsd antibody
      • Epididymis secretory sperm binding protein Li 130P antibody
      • HEL S 130P antibody
      • Lysosomal aspartyl peptidase antibody
      • Lysosomal aspartyl protease antibody
      • MGC2311 antibody
      see all

    Anti-Cathepsin D antibody [EPR3056Y] 图像

    • All lanes : Anti-Cathepsin D antibody [EPR3056Y] (ab75811) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : SKBR3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 46 kDa
      Observed band size : 15,46 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human liver using ab75811 at 1/500 dilution.

    Anti-Cathepsin D antibody [EPR3056Y] (ab75811)参考文献

    ab75811 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (small intestine, ganglion cells)
    Specification small intestine, ganglion cells
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, 100C 20 minutes
    Permeabilization No
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    提交于 Oct 22 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"