Anti-Cathepsin D抗体[CTD-19] (ab6313)

概述

  • 产品名称Anti-Cathepsin D抗体[CTD-19]
    参阅全部 Cathepsin D 一抗
  • 描述
    小鼠单克隆抗体[CTD-19] to Cathepsin D
  • 特异性Recognizes cathepsin D (34 kDa and weaker band at 52 kDa) in immunoblotting. Does not react with bovine cathepsin D and B, or with human cathepsin types B, C, G or H.
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IF, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Full length native protein (purified) (Human liver).

  • 阳性对照
    • Human breast carcinoma tissue or cell line extract.

性能

应用

Our Abpromise guarantee covers the use of ab6313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 52 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/200.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 24833013

靶标

  • 功能Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • 组织特异性Expressed in the aorta extrcellular space (at protein level).
  • 疾病相关Ceroid lipofuscinosis, neuronal, 10
  • 序列相似性Belongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • 翻译后修饰N- and O-glycosylated.
  • 细胞定位Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Information by UniProt
  • 数据库链接
  • 别名
    • CatD antibody
    • CATD_HUMAN antibody
    • Cathepsin D antibody
    • Cathepsin D heavy chain antibody
    • CD antibody
    • Ceroid lipofuscinosis neuronal 10 antibody
    • CLN10 antibody
    • CPSD antibody
    • ctsd antibody
    • Epididymis secretory sperm binding protein Li 130P antibody
    • HEL S 130P antibody
    • Lysosomal aspartyl peptidase antibody
    • Lysosomal aspartyl protease antibody
    • MGC2311 antibody
    see all

Anti-Cathepsin D antibody [CTD-19] 图像

  • Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/1000 dilution + MDA MB 231 whole cell lysate at 20 µg

    Secondary
    HRP-conjugated monoclonal sheep anti-mouse IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 28,48,50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 1 hour at 22°C.

    Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.

    See Abreview

  • ab6313 staining Cathepsin D in DU145 human prostate cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 and blocked with 5% BSA at room temperature for 20 minutes. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour. A CF488A-conjugated goat anti-mouse IgG (H+L) polyclonal was used as the secondary antibody (1/500).

    See Abreview

  • ab6313 staining Cathepsin D in HepaRG cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 in PBS and blocked with 1% milk at room temperature for 20 minutes. Samples were incubated with primary antibody (1µg/ml in 1% milk) for 30 minutes. An undiluted Alexa Fluor® 594-conjugated goat anti-mouse IgG  polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cells

Anti-Cathepsin D antibody [CTD-19] (ab6313)参考文献

This product has been referenced in:
  • Valdez HA  et al. Bordetella pertussis modulates human macrophage defense gene expression. Pathog Dis 74:N/A (2016). Read more (PubMed: 27465637) »
  • Tokuda E  et al. Low autophagy capacity implicated in motor system vulnerability to mutant superoxide dismutase. Acta Neuropathol Commun 4:6 (2016). WB ; Human . Read more (PubMed: 26810478) »

See all 15 Publications for this product

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (CSF)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification CSF
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 11 2015

Application Western blot
Sample Mouse Tissue lysate - whole (brain tissue)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification brain tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 11 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (10)
Sample Human Cell lysate - other (Glioblastoma cell lines)
Specification Glioblastoma cell lines
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Mr. Achilleas Mitrakas

Verified customer

提交于 Apr 25 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (DU145 Human prostate)
Specification DU145 Human prostate
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

提交于 Nov 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (adipocyte tissue)
Loading amount 35 µg
Specification adipocyte tissue
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 18 2012

Vielen Dank für Ihre Anfrage die Herr Hryca uns in den technischen Dienst weitergeleitet hat.

Ich habe geschaut, und wir haben viele möglichen positiven Kontrollen für Sie. Können Sie mir bitte noch sagen, welche Art von p...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HepaRG)
Specification HepaRG
Fixative Formaldehyde
Permeabilization Yes - Triton X-100 in PBS
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mrs. Alina Macovei

Verified customer

提交于 May 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepaRG)
Loading amount 10 µg
Specification HepaRG
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Mrs. Alina Macovei

Verified customer

提交于 May 03 2012

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