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Full length native protein (purified) (Human liver).
Our Abpromise guarantee covers the use of ab6313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 52 kDa.|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration. PubMed: 24833013|
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Blocked with 5% milk for 1 hour at 22°C.
Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.
ab6313 staining Cathepsin D in DU145 human prostate cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 and blocked with 5% BSA at room temperature for 20 minutes. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour. A CF488A-conjugated goat anti-mouse IgG (H+L) polyclonal was used as the secondary antibody (1/500).
ab6313 staining Cathepsin D in HepaRG cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 in PBS and blocked with 1% milk at room temperature for 20 minutes. Samples were incubated with primary antibody (1µg/ml in 1% milk) for 30 minutes. An undiluted Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.