概述

  • 产品名称Anti-Cathepsin D抗体
    参阅全部 Cathepsin D 一抗
  • 描述
    兔多克隆抗体to Cathepsin D
  • 特异性This antibody reacts with the zymogen as well as the activated form of cathepsin D. It stains fibroblasts and macrophages strongly but stains weakly with the myoepithelial cells of breast. It also stains sweat ducts and glands in the skin, islets cells and granules of pancreatic acini.
  • 经测试应用适用于: IHC-P, IHC-Fr, IHC-FoFr, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Cathepsin D isolated from human liver

  • 阳性对照
    • ICC/IF: A431 cells.

性能

应用

Our Abpromise guarantee covers the use of ab826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/75 - 1/100. ABC method.
IHC-Fr 1/75 - 1/100. ABC method.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19505575
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • 组织特异性Expressed in the aorta extrcellular space (at protein level).
  • 疾病相关Ceroid lipofuscinosis, neuronal, 10
  • 序列相似性Belongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • 翻译后修饰N- and O-glycosylated.
  • 细胞定位Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Information by UniProt
  • 数据库链接
  • 别名
    • CatD antibody
    • CATD_HUMAN antibody
    • Cathepsin D heavy chain antibody
    • CD antibody
    • Ceroid lipofuscinosis neuronal 10 antibody
    • CLN10 antibody
    • CPSD antibody
    • ctsd antibody
    • Epididymis secretory sperm binding protein Li 130P antibody
    • HEL S 130P antibody
    • Lysosomal aspartyl peptidase antibody
    • Lysosomal aspartyl protease antibody
    • MGC2311 antibody
    see all

Anti-Cathepsin D antibody 图像

  • ICC/IF image of ab826 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab826, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab826 staining Cathepsin D in formalin-fixed, paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • Immunohistochemical analysis of PFA-perfusion fixed, frozen rat brain tissue sections (substantia nigra), staining Cathepsin D with ab826. Samples were incubated with primary antibody overnight at room temperature.

Anti-Cathepsin D antibody (ab826)参考文献

This product has been referenced in:
  • Chu Y  et al. Alterations in lysosomal and proteasomal markers in Parkinson's disease: relationship to alpha-synuclein inclusions. Neurobiol Dis 35:385-98 (2009). IHC-FoFr ; Rat . Read more (PubMed: 19505575) »
  • Merseburger AS  et al. Cathepsin D expression in renal cell cancer-clinical implications. Eur Urol 48:519-26 (2005). IHC-P ; Human . Read more (PubMed: 16115525) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (brain)
Specification brain
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Oct 27 2014

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