Anti-Cathepsin D抗体(ab72915)


  • 产品名称Anti-Cathepsin D抗体
    参阅全部 Cathepsin D 一抗
  • 描述
    兔多克隆抗体to Cathepsin D
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Cathepsin D.

    (Peptide available as ab90605.)

  • 阳性对照
    • WB: MCF7, A431 and HepG2 whole cell lysates. ICC/IF: methanol fixed MCF-7 cells. IHC-P: human adrenal gland tissue.




Our Abpromise guarantee covers the use of ab72915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • 功能Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • 组织特异性Expressed in the aorta extrcellular space (at protein level).
  • 疾病相关Ceroid lipofuscinosis, neuronal, 10
  • 序列相似性Belongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • 翻译后修饰N- and O-glycosylated.
  • 细胞定位Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Information by UniProt
  • 数据库链接
  • 别名
    • CatD antibody
    • CATD_HUMAN antibody
    • Cathepsin D heavy chain antibody
    • CD antibody
    • Ceroid lipofuscinosis neuronal 10 antibody
    • CLN10 antibody
    • CPSD antibody
    • ctsd antibody
    • Epididymis secretory sperm binding protein Li 130P antibody
    • HEL S 130P antibody
    • Lysosomal aspartyl peptidase antibody
    • Lysosomal aspartyl protease antibody
    • MGC2311 antibody
    see all

Anti-Cathepsin D antibody 图像

  • IHC image of Cathepsin D staining in human adrenal gland formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72915, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Cathepsin D antibody (ab72915) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Predicted band size : 45 kDa
    Observed band size : 29,45,48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
    Cathepsin D has a predicted molecular weight of 45 kDa. The sequence contains a signal sequence and propeptide of 18 and 45 amino acids, respectively. This protein is further cleaved to produce a heavy and light chain with molecular weights of 27 kDa and 11 kDa, respectively (SwissProt). We hypothesize that the observed bands at 29 kDa represent the Cathepsin heavy chain, and the bands at 45 and 48 kDa represent the protein with and without the presence of the signal peptide.
  • ICC/IF image of ab72915 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72915 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Cathepsin D antibody (ab72915)参考文献

ab72915 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Cat Cell lysate - whole cell (CAT-MT and K12- Feline mammary carcinoma, SCCF1)
Gel Running Conditions Reduced Denaturing (4-20%)
Loading amount 50 µg
Specification CAT-MT and K12- Feline mammary carcinoma, SCCF1
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Holly Pondenis

Verified customer

提交于 Aug 26 2015