The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 46 kDa. The apparent mass may be increased due to glycosylation. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
功能Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
组织特异性Expressed in the aorta extrcellular space (at protein level).
疾病相关Ceroid lipofuscinosis, neuronal, 10
序列相似性Belongs to the peptidase A1 family. Contains 1 peptidase A1 domain.
翻译后修饰N- and O-glycosylated.
细胞定位Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
Epididymis secretory sperm binding protein Li 130P antibody
HEL S 130P antibody
Lysosomal aspartyl peptidase antibody
Lysosomal aspartyl protease antibody
Anti-Cathepsin D antibody 图像
Western blot - Cathepsin D antibody (ab49787)
All lanes : Anti-Cathepsin D antibody (ab49787) at 1/1000 dilution
Lane 1 : Recombinant Human Cathepsin D at 0.04 µg Lane 2 : Recombinant Human Cathepsin D at 0.02 µg Lane 3 : Recombinant Human Cathepsin D at 0.01 µg Lane 4 : Recombinant Human Cathepsin D at 0.004 µg Lane 5 : Recombinant Human Cathepsin D at 0.002 µg