概述

  • 产品名称Anti-Cathepsin D抗体[4G2]
    参阅全部 Cathepsin D 一抗
  • 描述
    小鼠单克隆抗体[4G2] to Cathepsin D
  • 经测试应用适用于: ELISA, WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 21-412 of Human Cathepsin D

  • 阳性对照
    • HepG2, Hep3B, MDA-MB-231 and MCF7 cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab40697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB 1/500 - 1/2000. Detects a band of approximately 45 kDa.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • 组织特异性Expressed in the aorta extrcellular space (at protein level).
  • 疾病相关Ceroid lipofuscinosis, neuronal, 10
  • 序列相似性Belongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • 翻译后修饰N- and O-glycosylated.
  • 细胞定位Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Information by UniProt
  • 数据库链接
  • 别名
    • CatD antibody
    • CATD_HUMAN antibody
    • Cathepsin D heavy chain antibody
    • CD antibody
    • Ceroid lipofuscinosis neuronal 10 antibody
    • CLN10 antibody
    • CPSD antibody
    • ctsd antibody
    • Epididymis secretory sperm binding protein Li 130P antibody
    • HEL S 130P antibody
    • Lysosomal aspartyl peptidase antibody
    • Lysosomal aspartyl protease antibody
    • MGC2311 antibody
    see all

Anti-Cathepsin D antibody [4G2] 图像

  • Overlay histogram showing MCF7 cells stained with ab40697 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40697, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-Cathepsin D antibody [4G2] (ab40697) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Hep3B cell lysate
    Lane 3 : MDA-MB-231 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique

    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

Anti-Cathepsin D antibody [4G2] (ab40697)参考文献

This product has been referenced in:
  • McNeill A  et al. Ambroxol improves lysosomal biochemistry in glucocerebrosidase mutation-linked Parkinson disease cells. Brain 137:1481-95 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24574503) »
  • Shen C  et al. Global profiling of proteolytically modified proteins in human metastatic hepatocellular carcinoma cell lines reveals CAPN2 centered network. Proteomics 12:1917-27 (2012). Human . Read more (PubMed: 22623320) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (hepatocytes)
Specification hepatocytes
Fixative Formaldehyde
Permeabilization Yes - triton X-100 0.1%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Verified customer

提交于 May 28 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2)
Loading amount 25 µg
Specification HepG2
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

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提交于 May 27 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"